Langley O K, Aletsee-Ufrecht M C, Grant N J, Gratzl M
INSERM U44, Centre de Neurochimie du CNRS, Strasbourg, France.
J Histochem Cytochem. 1989 Jun;37(6):781-91. doi: 10.1177/37.6.2723399.
We examined the expression of the neural cell adhesion molecule NCAM in a number of endocrine tissues of adult rat and in an endocrine tumor cell line. NCAM was found by immunoelectron microscopy to be present on the surface of all endocrine cells in the three lobes of the hypophysis, although staining was relatively less intense in the intermediate lobe, and in pancreatic islets. Pituicytes, hypophyseal glial cells, were also labeled for NCAM. A rat insulinoma cell line (RIN A2) also expressed NCAM as judged by immunocytochemistry. Analysis of NCAM antigenic determinants (Mr 180, 140, and 120 KD) revealed large variations in the relative proportions of NCAM polypeptides present in the different tissues. Although all tissues and cell lines expressed NCAM-140, NCAM-180 was not detected in the adenohypophysis, pancreas, or adrenal medulla, and NCAM-120 was found in none of the endocrine tissues or cell lines except at low levels in the neurohypophysis. The tumor cell line expressed significant levels of NCAM-180, which was most abundant in the neurohypophysis. These results show that NCAM expression appears to be a general property of endocrine cells, although the antigenic composition differs markedly from that in brain tissue. These data are discussed with regard to the embryological origins of the different endocrine tissues, and possible functional implications are suggested.
我们检测了成年大鼠多种内分泌组织及一种内分泌肿瘤细胞系中神经细胞黏附分子NCAM的表达情况。通过免疫电子显微镜发现，NCAM存在于垂体三个叶的所有内分泌细胞表面，尽管中间叶和胰岛的染色相对较弱。垂体细胞，即垂体神经胶质细胞，也被标记为NCAM阳性。通过免疫细胞化学判断，大鼠胰岛素瘤细胞系（RIN A2）也表达NCAM。对NCAM抗原决定簇（分子量180、140和120kD）的分析显示，不同组织中存在的NCAM多肽相对比例差异很大。尽管所有组织和细胞系都表达NCAM-140，但在腺垂体、胰腺或肾上腺髓质中未检测到NCAM-180，除了在神经垂体中含量较低外，在其他内分泌组织或细胞系中均未发现NCAM-120。肿瘤细胞系表达了高水平的NCAM-180，其在神经垂体中最为丰富。这些结果表明，NCAM的表达似乎是内分泌细胞的一个普遍特性，尽管其抗原组成与脑组织明显不同。本文结合不同内分泌组织的胚胎学起源对这些数据进行了讨论，并提出了可能的功能意义。
