蛋白质棕榈酰化在母源-合子转变过程中激活合子基因表达。
Protein palmitoylation activate zygotic gene expression during the maternal-to-zygotic transition.
作者信息
Du Zhaoxia, Chen Xueran, Li Xian, He Kun, Ji Shufang, Shi Wei, Hao Aijun
机构信息
Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong Provincial Key Laboratory of Mental Disorders, Department of Histology and Embryology, Shandong University School of Medicine, No. 44, Wenhua Xi Road, Jinan, Shandong, 250012, PR China.
Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, No. 350, Shushan Hu Road, Hefei, Anhui, 230031, PR China.
出版信息
Biochem Biophys Res Commun. 2016 Jun 24;475(2):194-201. doi: 10.1016/j.bbrc.2016.05.074. Epub 2016 May 24.
Upon fertilization, maternal factors direct development and trigger zygotic genome activation at the maternal-to-zygotic transition (MZT). However, the factors that activate the zygotic program in vertebrates are not well defined. Here, we found that protein palmitoylation played an important role in acquiring transcriptional competency and orchestrating the clearance of the maternal program in zebrafish. After inhibition of protein palmitoylation, zebrafish embryos developed normally before the Mid-Blastula Transition (MBT); however, they did not initiate epiboly. Moreover, our results showed that protein palmitoylation is required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression.
受精时，母体因素指导发育并在母型向合子型转变（MZT）时触发合子基因组激活。然而，脊椎动物中激活合子程序的因素尚未明确界定。在这里，我们发现蛋白质棕榈酰化在获得转录能力和协调斑马鱼母体程序清除方面发挥了重要作用。抑制蛋白质棕榈酰化后，斑马鱼胚胎在中囊胚转换（MBT）之前发育正常；然而，它们并未开始外包。此外，我们的结果表明，蛋白质棕榈酰化是启动合子发育程序并通过激活miR-430表达诱导母体程序清除所必需的。
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