Suppr超能文献

新型氧化石墨烯固定化酿酒酵母凝胶珠对铀的去除

Uranium removal by novel graphene oxide-immobilized Saccharomyces cerevisiae gel beads.

作者信息

Chen Can, Wang Jianlong

机构信息

Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, PR China.

Collaborative Innovation Center for Advanced Nuclear Energy Technology, INET, Tsinghua University, Beijing, 100084, PR China; Beijing Key Laboratory of Radioactive Waste Treatment, Tsinghua University, Beijing, 100084, PR China.

出版信息

J Environ Radioact. 2016 Oct;162-163:134-145. doi: 10.1016/j.jenvrad.2016.05.012. Epub 2016 May 25.

Abstract

To evaluate its ability to absorb dissolved uranium (VI), the waste biomass of Saccharomyces cerevisiae was immobilized using different agents, including Ca-alginate (Ca-SA), Ca-alginate with graphene oxide (GO), polyvinyl alcohol (PVA, 5% or 10%, w/v)-SA-GO in CaCl-boric acid solution. The experimental results showed that graphene oxide at 0.01% (w/v) could enhance the performance of the immobilized cells. The yeast gel beads prepared with 5% PVA-1% SA-2% yeast-0.01% GO-2% CaCl-saturated boric acid (4#) generally showed the better physical-chemical properties such as higher tolerance to the unfavorable environmental conditions. Moreover, the 4# gel beads exhibited more stable capacity for U(VI) sorption and desorption at various conditions, such as pH in the range of 3-9. A pseudo second-order kinetic model could describe the kinetics of U(VI) sorption onto the 4# gel beads (R = 0.96). The Langmuir, Freundlich, Tempkin and Sips models could be used to describe U(VI) sorption by the 4# gel beads, with the R being 0.90, 0.83, 0.96, 0.97, respectively. The Sips maximum capacity of 4# gel beads was 24.4 mg U/g dry weight. The desorption efficiency of U(VI) adsorbed onto the 4# gel beads was 91%, 73% and 40% by 0.1 M HNO, 0.1 M HCl and 0.1 M NaOH, respectively. However, the 4# gel beads exhibited lower U(VI) sorption capacity than the raw yeast cell (Sips maximum capacity of 35.6 mg U/g). The immobilized Saccharomyces cerevisiae using SA, PVA and/or GO showed obvious changes in the molecular vibration of functional groups such as carboxyl, amide and hydroxyl groups compared with the raw yeast cells, according to FTIR analysis. The SEM-EDX analysis showed that U(VI) was adsorbed unevenly on the cellular surface. Carboxyl and hydroxyl groups may be involved in U(VI) binding by yeast cells.

摘要

为评估酿酒酵母废弃生物质吸附溶解态铀(VI)的能力,使用不同试剂对其进行固定化处理,包括海藻酸钙(Ca-SA)、含氧化石墨烯(GO)的海藻酸钙、在氯化钙-硼酸溶液中的聚乙烯醇(PVA,5%或10%,w/v)-SA-GO。实验结果表明,0.01%(w/v)的氧化石墨烯可提高固定化细胞的性能。用5% PVA-1% SA-2%酵母-0.01% GO-2%氯化钙-饱和硼酸制备的酵母凝胶珠(4#)通常表现出更好的物理化学性质,如对不利环境条件的耐受性更高。此外,4#凝胶珠在各种条件下,如pH值在3至9范围内,对U(VI)的吸附和解吸能力更稳定。准二级动力学模型可描述U(VI)在4#凝胶珠上的吸附动力学(R = 0.96)。Langmuir、Freundlich、Tempkin和Sips模型可用于描述4#凝胶珠对U(VI)的吸附,R值分别为0.90、0.83、0.96、0.97。4#凝胶珠的Sips最大吸附量为24.4 mg U/g干重。吸附在4#凝胶珠上的U(VI)分别用0.1 M HNO₃、0.1 M HCl和0.1 M NaOH解吸时,解吸效率分别为91%、73%和40%。然而,4#凝胶珠的U(VI)吸附能力低于原始酵母细胞(Sips最大吸附量为35.6 mg U/g)。根据傅里叶变换红外光谱(FTIR)分析,与原始酵母细胞相比,使用SA、PVA和/或GO固定化的酿酒酵母在羧基、酰胺基和羟基等官能团的分子振动方面表现出明显变化。扫描电子显微镜-能谱分析(SEM-EDX)表明,U(VI)在细胞表面的吸附不均匀。羧基和羟基可能参与酵母细胞对U(VI)的结合。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验