Genetic Modification of Human Pancreatic Progenitor Cells Through Modified mRNA.
作者信息
Lu Song, Chow Christie C, Zhou Junwei, Leung Po Sing, Tsui Stephen K, Lui Kathy O
机构信息
Department of Chemical Pathology, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China.
Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China.
出版信息
Methods Mol Biol. 2016;1428:307-17. doi: 10.1007/978-1-4939-3625-0_21.
In this chapter, we describe a highly efficient genetic modification strategy for human pancreatic progenitor cells using modified mRNA-encoding GFP and Neurogenin-3. The properties of modified mRNA offer an invaluable platform to drive protein expression, which has broad applicability in pathway regulation, directed differentiation, and lineage specification. This approach can also be used to regulate expression of other pivotal transcription factors during pancreas development and might have potential therapeutic values in regenerative medicine.
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