Umar Sajid, Younus Muhammad, Shahzad Muhammad, Aqil Kiran, Qayyum Rizwan, Mushtaq Aqsa, Shah Muhammad Ali Abdullah, Munir Muhammad Tanveer
National Veterinary School of Toulouse, Toulouse, France; Department of Pathobiology, Faculty of Veterinary Sciences, University of Arid Agriculture, Rawalpindi, Pakistan.
Department of Pathobiology, College of Veterinary & Animal Sciences, Jhang, Pakistan.
Scientifica (Cairo). 2016;2016:4381067. doi: 10.1155/2016/4381067. Epub 2016 Apr 28.
The study was conducted to investigate the effects of wheat based diet on the pathology of necrotic enteritis in turkeys. Turkeys were divided into four groups. Groups A and B were kept as noninoculated and fed normal commercial diet while groups C and D were challenged orally with C. perfringens and fed wheat based diet to promote the development of experimental disease. Infected turkeys showed clinical signs of depression, ruffled feathers, and dark yellowish faeces showing the most prominent disease signs in turkeys of group D with 30% mortality. Similarly, turkeys of group D showed more striking gross and histopathologic lesions as compared to turkeys of group C. The most severe gross lesions comprised intestinal distension, small necrotic spots and haemorrhages on intestine, fragile intestinal wall, and gas bubble formation in the small intestine. Histologically, inoculated turkeys showed patchy necrosis, desquamation of intestinal epithelium, and intense leukocyte infiltration in the intestine. Microscopic examination showed significant decrease in the height of intestinal villi of inoculated birds. Haematological studies showed significant influence of necrotic enteritis on the blood profile of turkeys in group D. The findings revealed that simultaneous feeding of wheat enhanced the pathology of necrotic enteritis in turkeys.
本研究旨在调查以小麦为基础的日粮对火鸡坏死性肠炎病理学的影响。将火鸡分为四组。A组和B组不接种，饲喂正常商业日粮，而C组和D组经口服产气荚膜梭菌攻毒，并饲喂以小麦为基础的日粮以促进实验性疾病的发展。感染的火鸡表现出抑郁、羽毛蓬乱和深黄色粪便等临床症状，其中D组火鸡的疾病症状最为明显，死亡率达30%。同样，与C组火鸡相比，D组火鸡表现出更明显的大体和组织病理学病变。最严重的大体病变包括肠道扩张、小肠坏死小点和出血、肠壁脆弱以及小肠内气泡形成。组织学上，接种的火鸡表现出局灶性坏死、肠上皮细胞脱落以及肠道内大量白细胞浸润。显微镜检查显示，接种火鸡的肠绒毛高度显著降低。血液学研究表明，坏死性肠炎对D组火鸡的血液指标有显著影响。研究结果显示，同时饲喂小麦会加重火鸡坏死性肠炎的病理学变化。
