Naseripour Masood, Mehrazma Mitra, Pourmatin Rama, Kashkouli Mohsen Bahmani, Sedaghat Ahad, Gheytanchi Elmira
Eye Research Center, Rassoul Akram Hospital, Ophthalmology Department, Iran University of Medical Sciences [IUMS], Tehran, Iran.
Oncopathology Research Center, Rassoul Akram Hospital, Iran University of Medical Sciences [IUMS], Tehran, Iran.
J Curr Ophthalmol. 2015 Nov 4;27(1-2):41-5. doi: 10.1016/j.joco.2015.09.002. eCollection 2015 Mar-Jun.
The aim of this study was to investigate the rate of monosomy 3 by CISH technique in Iranian patients with uveal melanoma (UM) and its correlation with clinical and histopathological features.
Archival formalin fixed, paraffin-embedded material from 50 patients who had undergone enucleation for large uveal melanomas was obtained. Monosomy of chromosome 3 alteration by chromogenic in situ hybridization (CISH) was investigated. Clinical and histopathological features of tumors were collected.
The patients had a mean age of 56.6±7.6 years. Mean basal diameter and thickness of tumors were 14.1 mm and 10.2 mm, respectively. Four patients (8%) were identified to harbor monosomy of chromosome 3. In the mean follow-up of 5.3 years (range, 3.2-9.5 y), only one case with monosomy 3 died of UM metastasis. The most common type of cellularity was mixed cell (86%). There was not any statistically significant correlation between monosomy of chromosome 3 and type of cellularity, ciliary body involvement, and largest basal diameter.
The low rate of monosomy chromosome 3 and the consequent low rate of mortality may be indicative of good prognosis in Iranian patients with uveal melanoma.
本研究旨在通过显色原位杂交（CISH）技术调查伊朗葡萄膜黑色素瘤（UM）患者中3号染色体单体的发生率及其与临床和组织病理学特征的相关性。
获取50例因大葡萄膜黑色素瘤而接受眼球摘除术患者的存档福尔马林固定、石蜡包埋材料。通过显色原位杂交（CISH）研究3号染色体改变的单体情况。收集肿瘤的临床和组织病理学特征。
患者的平均年龄为56.6±7.6岁。肿瘤的平均基底直径和厚度分别为14.1毫米和10.2毫米。4例（8%）患者被鉴定为存在3号染色体单体。在平均5.3年（范围3.2 - 9.5年）的随访中，仅1例3号染色体单体患者死于UM转移。最常见的细胞类型是混合细胞（86%）。3号染色体单体与细胞类型、睫状体受累情况和最大基底直径之间无统计学显著相关性。
3号染色体单体的低发生率以及随之而来的低死亡率可能表明伊朗葡萄膜黑色素瘤患者预后良好。
