Yildirim Ahmet, Yildirim Sule, Topaloglu Naci, Tekin Mustafa, Kucuk Adem, Erdem Havva, Erbas Mesut, Cakir Dilek Ulker
Department of Emergency Medicine, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
Department of Pediatrics, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
Turk J Emerg Med. 2016 Mar 10;16(1):8-11. doi: 10.1016/j.tjem.2016.02.004. eCollection 2016 Mar.
Ischemia modified albumin (IMA) levels significantly increased and may be used as a diagnostic marker in ovarian torsion. The aim of this study is to investigate whether there was any correlation between IMA levels and histopathologic changes in experimental ovarian torsion.
Fourteen Sprague-Dawley rats, each weighing 220-250 g were divided randomly into 2 groups; in Group 1, the control group (n = 7), only laparotomy was performed and in Group 2, the experimental group (n = 7), ovarian torsion was performed. Ischemia was performed for 3 h; following the ischemia period, the torsion was relieved by detwisting the adnexa and then the ovarian I/R protocol was applied for 3 h. Blood samples were taken from all of the rats to measure the IMA levels and the ovaries were surgically removed for histologic examination. A blinded pathologist examined and scored the samples.
The median (minimum-maximum) IMA values were 921.00 (870.00-966.00) ABSUs in the ovarian torsion group and 853.00 (782.00-869.00) ABSUs in the control group. The difference was statistically significant. In the correlation analysis, a significant and strong correlation was found between IMA levels and histopathologic changes (Spearman's rho = +0.987, p < 0.001).
Positive correlation was found between the IMA levels and the histopathologic severity of the disease. This finding is important for both diagnosis of the disease and patient follow-up. As a new marker in ovarian torsion, IMA may also indicate the severity of the ovarian histopathology.
缺血修饰白蛋白（IMA）水平显著升高，可作为卵巢扭转的诊断标志物。本研究旨在探讨实验性卵巢扭转中IMA水平与组织病理学变化之间是否存在相关性。
14只体重220 - 250克的Sprague-Dawley大鼠随机分为2组；第1组为对照组（n = 7），仅行剖腹术；第2组为实验组（n = 7），进行卵巢扭转。缺血3小时；缺血期过后，通过扭转附件解除扭转，然后进行3小时的卵巢缺血再灌注方案。采集所有大鼠的血样以测量IMA水平，并手术切除卵巢进行组织学检查。由一位不知情的病理学家对样本进行检查和评分。
卵巢扭转组的IMA值中位数（最小值 - 最大值）为921.00（870.00 - 966.00）ABSU，对照组为853.00（782.00 - 869.00）ABSU。差异具有统计学意义。在相关性分析中，发现IMA水平与组织病理学变化之间存在显著且强的相关性（Spearman秩相关系数 = +0.987，p < 0.001）。
发现IMA水平与疾病的组织病理学严重程度呈正相关。这一发现对疾病的诊断和患者随访都很重要。作为卵巢扭转的一种新标志物，IMA也可能指示卵巢组织病理学变化的严重程度。
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