Gencer Meryem, Karaca Turan, Güngör Ayşe N C, Hacıvelioğlu Servet Ö, Demirtaş Selim, Turkon Hakan, Uysal Ahmet, Korkmaz Fatma, Coşar Emine, Hancı Volkan
Çanakkale Onsekiz Mart University, Medical Faculty, Department of Obstetrics and Gynecology, Çanakkale, Turkey.
Trakya University, Medical Faculty, Department of Histology and Embryology, Edirne, Turkey.
Eur J Obstet Gynecol Reprod Biol. 2014 Jun;177:135-40. doi: 10.1016/j.ejogrb.2014.03.036. Epub 2014 Apr 13.
To investigate the protective effect of quercetin (QE), an anti-inflammatory and anti-oxidant agent, on torsion-detorsion induced histopathological changes and blood IMA levels in experimental ovarian ischemia-reperfusion (IR) injury.
Twenty-four female Wistar rats were randomly divided into four groups in this study (n=6). Group I, (sham operation); Group II, torsion-detorsion plus saline (IR); Group III, torsion-detorsion plus solvent (dimethylsulfoxide: DMSO, IR+DMSO); Group IV, torsion-detorsion plus 15 mg/kg/bw quercetin (IR+QE) injected intraperitoneally 30 min prior to detorsion. After 3h of reperfusion, the right ovaries were removed surgically. The ovary tissue samples were fixed in 10% formalin solution for histopathological and immunohistochemical examination. Blood samples were obtained at the end of the procedures for each group of animals.
Ovarian sections in Groups II and III showed higher follicular cell degeneration, hemorrhage, vascular congestion and edema when compared with Group I. Administration of quercetin in rats significantly prevented degenerative changes in the ovary. Significantly less histopathological changes were found in Group IV compared with Groups II and III. Caspase-3 and TUNEL positive cells were detected in the ovarian surface, follicle epithelium, and stromal cells in all experimental groups, and there was a significant increase in Groups II and III compared with Group I (P<0.05). Treatment with quercetin decreased the number of caspase-3 and TUNEL positive cells. IR increased the ischemia modified albumin (IMA) levels in comparison to the sham group (1.06 ± 0.10 ABSU and 0.92 ± 0.08 ABSU, P<0.05). Quercetin administration before IR reduced the levels of IMA (0.93 ± 0.08 ABSU, P<0.05).
Administration of quercetin is effective in preventing tissue damage induced by IR injury in ovaries.
研究抗炎抗氧化剂槲皮素（QE）对实验性卵巢缺血再灌注（IR）损伤中扭转-复位诱导的组织病理学变化和血液缺血修饰白蛋白（IMA）水平的保护作用。
本研究将24只雌性Wistar大鼠随机分为四组（n = 6）。第一组（假手术组）；第二组，扭转-复位加生理盐水（IR组）；第三组，扭转-复位加溶剂（二甲基亚砜：DMSO，IR + DMSO组）；第四组，扭转-复位加15 mg/kg体重的槲皮素（IR + QE组），在复位前30分钟腹腔注射。再灌注3小时后，手术切除右侧卵巢。卵巢组织样本固定于10%福尔马林溶液中进行组织病理学和免疫组织化学检查。在每组动物实验结束时采集血样。
与第一组相比，第二组和第三组的卵巢切片显示卵泡细胞变性、出血、血管充血和水肿更严重。给大鼠施用槲皮素可显著预防卵巢的退行性变化。与第二组和第三组相比，第四组的组织病理学变化明显更少。在所有实验组的卵巢表面、卵泡上皮和基质细胞中均检测到半胱天冬酶-3（Caspase-3）和TUNEL阳性细胞，与第一组相比，第二组和第三组显著增加（P < 0.05）。槲皮素治疗减少了Caspase-3和TUNEL阳性细胞的数量。与假手术组相比，IR增加了缺血修饰白蛋白（IMA）水平（分别为1.06 ± 0.10 ABSU和0.92 ± 0.08 ABSU，P < 0.05）。IR前施用槲皮素降低了IMA水平（0.93 ± 0.08 ABSU，P < 0.05）。
施用槲皮素可有效预防卵巢IR损伤诱导的组织损伤。
