Altaeva A Zh, Galitskiy F A, Zhakupova T Z, Aidarkulov A Sh, Selivokhina N V, Zhunisov S S
Centre of Forensic Medicine, Kazakh Ministry of Justice, Almaty, Republic of Kazakhstan, 050000.
Astana Medical University, Kazakh Ministry of Health, Astana, Republic of Kazakhstan, 010000.
Sud Med Ekspert. 2016 May-Jun;59(3):12-15. doi: 10.17116/sudmed201659312-15.
The objective of the present study was to improve forensic medical diagnostics of the cases of death associated with morphine poisoning based on the investigation into the biochemical changes in blood and pericardial fluid as well as morphological changes in the myocardial structures. The studies were carried out with the use of thin-layer chromatography, colorimetric and morphological methods including hematoxylin and eosin, Lee's methylene blue, and van Gieson's picrofuscin staining. These techniques were supplemented by light and polarization microscopy. The study has demonstrated the presence of morphine in 99.16% of the blood and pericardial samples obtained in the cases of poisoning. The comparison of the results of biochemical and pathomorphological studies of the myocardium made it possible to evaluate the functional and morphological conditions of the heart in the case of acute morphine poisoning during the period of chronic drug intoxication.
本研究的目的是通过调查血液和心包液中的生化变化以及心肌结构的形态变化，改进与吗啡中毒相关死亡案例的法医学诊断。研究采用薄层色谱法、比色法和形态学方法，包括苏木精和伊红染色、李氏亚甲蓝染色和范吉森氏苦味酸复红染色。这些技术辅以光学显微镜和偏光显微镜。研究表明，在中毒案例中获得的99.16%的血液和心包样本中存在吗啡。对心肌的生化和病理形态学研究结果进行比较，有助于评估慢性药物中毒期间急性吗啡中毒情况下心脏的功能和形态状况。
