Şekeroğlu Vedat, Aksoy Muhammet, Atlı Şekeroğlu Zülal
a Department of Biology , Faculty of Science and Letters, Ordu University , Ordu , Turkey.
Drug Chem Toxicol. 2017 Apr;40(2):140-145. doi: 10.1080/01480545.2016.1188305. Epub 2016 May 31.
Ofloxacin (OFX), a second-generation of quinolones, is a broad-spectrum flouroquinolone antibiotic used in the treatment of various bacterial infections. In this article, we aimed to investigate the cytotoxic and genotoxic potentials of OFX in cultured human peripheral lymphocytes. The cytotoxicity and genotoxicity of OFX on human peripheral blood lymphocytes were examined in vitro by sister chromatid exchange (SCE), chromosomal aberrations (CAs) and micronucleus (MN) tests. Cultures were treated with 30, 60 and 120 μg/ml of OFX for 48 h. Dimethylsulfoxide (DMSO) was used as a solvent control. OFX decreased the mitotic index (MI) and nuclear division index (NDI) significantly, especially at higher concentrations (60 and 120 μg/ml) compared with solvent control. OFX significantly induced CAs at all concentrations and SCEs at higher concentrations (60 and 120 μg/ml) compared with solvent control. In conclusion, our results indicated that OFX has cytotoxic, cytostatic and genotoxic potential especially at higher concentrations on human peripheral blood lymphocyte cultures under the experimental conditions.
氧氟沙星（OFX）是第二代喹诺酮类药物，是一种广谱氟喹诺酮类抗生素，用于治疗各种细菌感染。在本文中，我们旨在研究OFX对培养的人外周血淋巴细胞的细胞毒性和遗传毒性潜力。通过姐妹染色单体交换（SCE）、染色体畸变（CAs）和微核（MN）试验，在体外检测了OFX对人外周血淋巴细胞的细胞毒性和遗传毒性。将培养物用30、60和120μg/ml的OFX处理48小时。使用二甲基亚砜（DMSO）作为溶剂对照。与溶剂对照相比，OFX显著降低了有丝分裂指数（MI）和核分裂指数（NDI），尤其是在较高浓度（60和120μg/ml）时。与溶剂对照相比，OFX在所有浓度下均显著诱导CAs，在较高浓度（60和120μg/ml）时诱导SCE。总之，我们的结果表明，在实验条件下，OFX尤其是在较高浓度时对人外周血淋巴细胞培养物具有细胞毒性、细胞抑制和遗传毒性潜力。
