从桑椹（Morus alba L.）中分离得到的一种新型多糖及其硒化物衍生物：结构表征与生物活性
A novel polysaccharide isolated from mulberry fruits (Murus alba L.) and its selenide derivative: structural characterization and biological activities.
作者信息
Chen Chun, Zhang Bin, Fu Xiong, Liu Rui Hai
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
出版信息
Food Funct. 2016 Jun 15;7(6):2886-97. doi: 10.1039/c6fo00370b. Epub 2016 May 31.
A novel polysaccharide (MFP3P) was isolated from Murus alba L. through the hot water extraction method followed by chromatographic purification. The chemical structure of MFP3P was elucidated by acid hydrolysis, Smith degradation and methylation analysis, along with FT-IR, GC-MS, (1)H and (13)C NMR spectroscopy. Its morphological properties were further characterized by SEM and AFM. The selenide of the polysaccharide (MFP3P-Se) was obtained by the Na2SeO3/BaCl2 method. The antioxidant properties showed that MFP3P-Se exhibited higher peroxy radical-scavenging capacity than MFP3P in vitro. Moreover, MFP3P-Se had more significant hypoglycemic effects than MFP3P through promoting pancreatic cell proliferation and increasing glucose metabolism and insulin secretion.
通过热水提取法继以色谱纯化，从白桑（Morus alba L.）中分离出一种新型多糖（MFP3P）。通过酸水解、Smith降解和甲基化分析，以及傅里叶变换红外光谱（FT-IR）、气相色谱-质谱联用（GC-MS）、氢核磁共振（¹H NMR）和碳核磁共振（¹³C NMR）光谱对MFP3P的化学结构进行了阐明。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）进一步表征了其形态学性质。采用亚硒酸钠/氯化钡法获得了该多糖的硒化物（MFP3P-Se）。抗氧化性能表明，MFP3P-Se在体外表现出比MFP3P更高的过氧自由基清除能力。此外，MFP3P-Se通过促进胰腺细胞增殖、增加葡萄糖代谢和胰岛素分泌，比MFP3P具有更显著的降血糖作用。
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