Nomura Yasuya, Kimura Yurika, Kobayashi Hitome
a Department of Otorhinolaryngology , Showa University , Tokyo , Japan.
Acta Otolaryngol. 2016 Nov;136(11):1193-1195. doi: 10.1080/00016489.2016.1185541. Epub 2016 May 31.
The authors would like to confirm a fluid pathway from the scala tympani to the organ of Corti, and to observe its morphological changes.
A staining solution for succinic dehydrogenase was perfused with phenazine methosulfate in the scala tympani of living guinea pigs (n = 5) under deep anesthesia. After fixation, the cochleas were eventually embedded in epon. Sections were observed under a light microscope.
Blue-stained tissue is indicative of the pathway taken by the solution. The staining solution entered the organ of Corti through Hensen-Deiters' slit. The slit widened and Hensen's cells were pushed laterally. A new space was formed medial to Hensen's cells. Cortilymphatic hydrops developed.
The Hensen-Deiters' slit is a pathway of a certain staining solution from the scala tympani to inside the organ of Corti of the guinea pig. The influx of the fluid pushes Hensen's cells laterally and upward, resulting in a formation of hydrops of the organ of Corti or cortilymphatic hydrops. The hydrops is observed in animals with experimental perilymphatic fistula and with viral labyrinthitis. At the end stage of the hydrops, only the surface of the organ of Corti remains as a thin layer without any cellular elements.
作者希望证实从鼓阶到柯蒂氏器的液体通路，并观察其形态变化。
在深度麻醉下，将含有吩嗪硫酸甲酯的琥珀酸脱氢酶染色液灌注到活体豚鼠（n = 5）的鼓阶中。固定后，将耳蜗最终包埋在环氧树脂中。在光学显微镜下观察切片。
蓝色染色组织表明溶液所经过的通路。染色液通过亨森 - 代特间隙进入柯蒂氏器。该间隙变宽，亨森氏细胞被推向外侧。在亨森氏细胞内侧形成了一个新的空间。出现了 Cortilymphatic 积水。
亨森 - 代特间隙是某种染色液从鼓阶进入豚鼠柯蒂氏器内部的一条通路。液体的流入将亨森氏细胞向外和向上推，导致柯蒂氏器积水或 Cortilymphatic 积水的形成。在实验性外淋巴瘘和病毒性迷路炎的动物中观察到了积水。在积水的末期，仅柯蒂氏器的表面保留为一层没有任何细胞成分的薄层。
