2002年至2012年在俄罗斯远东地区自由放养的东北虎（Panthera tigris altaica）身上采集的蜱虫中检测到猫肝簇虫

Detection of Hepatozoon felis in Ticks Collected from Free-Ranging Amur Tigers ( Panthera tigris altaica), Russian Far East, 2002-12.

作者信息

Thomas Lindsay H, Seryodkin Ivan V, Goodrich John M, Miquelle Dale G, Birtles Richard J, Lewis John C M

机构信息

1 Manor Vets, 373 Hagley Road, Edgbaston, Birmingham B17 8DL, UK;

2 Pacific Geographical Institute Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690041, Russia;

出版信息

J Wildl Dis. 2016 Jul;52(3):674-6. doi: 10.7589/2015-10-277. Epub 2016 May 31.

DOI:10.7589/2015-10-277

PMID:27243154

Abstract

We collected 69 ticks from nine, free-ranging Amur tigers ( Panthera tigris altaica) between 2002 and 2011 and investigated them for tick-borne pathogens. DNA was extracted using alkaline digestion and PCR was performed to detect apicomplexan organisms. Partial 18S rDNA amplification products were obtained from 14 ticks from four tigers, of which 13 yielded unambiguous nucleotide sequence data. Comparative sequence analysis revealed all 13 partial 18S rDNA sequences were most similar to those belonging to strains of Hepatozoon felis (>564/572 base-pair identity, >99% sequence similarity). Although this tick-borne protozoon pathogen has been detected in wild felids from many parts of the world, this is the first record from the Russian Far East.

摘要

2002年至2011年间，我们从9只自由放养的东北虎（Panthera tigris altaica）身上采集了69只蜱虫，并对其进行蜱传病原体调查。采用碱性消化法提取DNA，并进行PCR检测顶复门生物。从4只老虎身上的14只蜱虫中获得了部分18S rDNA扩增产物，其中13只产生了明确的核苷酸序列数据。比较序列分析显示，所有13个部分18S rDNA序列与猫肝簇虫（Hepatozoon felis）菌株的序列最为相似（>564/572碱基对同一性，>99%序列相似性）。尽管这种蜱传原生动物病原体已在世界许多地区的野生猫科动物中被检测到，但这是俄罗斯远东地区的首次记录。