French G L, Talsania H, Phillips I
Department of Microbiology, United Medical School of Guy's, St Thomas's Campus, London.
J Med Microbiol. 1989 May;29(1):19-27. doi: 10.1099/00222615-29-1-19.
An isothermal method of pyrolysis-gas chromatography (Py-GC) was used for the identification of viridans streptococci. Pyrograms from 104 reference strains were subjected to a discriminant analysis to produce classification coefficients for the identification of 74 test organisms. Five groups representing recognised species were discriminated but Streptococcus milleri strains could not be distinguished from S. sanguis. If S. milleri and S. sanguis are regarded as a single pyrogroup, only three strains out of 74 were incorrectly identified by Py-GC. A multidimensional scaling analysis of the Py-GC data produced a similar species grouping, but this statistical method was less satisfactory for pyrogram data than discriminant analysis. While Py-GC was moderately successful for the identification of viridans streptococci, this study indicated that the technique has limited use in diagnostic medical microbiology because it is time-consuming and lacks flexibility.
采用热解气相色谱（Py-GC）等温法鉴定草绿色链球菌。对104株参考菌株的热解图谱进行判别分析，以生成用于鉴定74株测试菌株的分类系数。区分出了代表公认菌种的五组，但米勒链球菌菌株无法与 sanguis链球菌区分开来。如果将米勒链球菌和 sanguis链球菌视为一个单一的热解群组，那么在74株菌株中，热解气相色谱法仅错误鉴定了3株。对热解气相色谱数据进行的多维标度分析产生了类似的菌种分组，但这种统计方法对热解图谱数据的适用性不如判别分析。虽然热解气相色谱法在鉴定草绿色链球菌方面取得了一定成功，但这项研究表明，该技术在诊断医学微生物学中的应用有限，因为它耗时且缺乏灵活性。
