Oka S, Wada Y, Yamazaki N, Yamashita T, Kumazawa T, Kawamoto K
Dept. of Otorrhinolaryngology, Kansai Medical Univ.
Gan No Rinsho. 1989 May;35(6):653-8.
Using flow cytometry, basic experiments and clinical trials to investigate DNA aneuploidy were performed. The site of the peak in the DNA histogram was found to be unstable in the flow cytometric assay. However, relatively stable DNA indices can be obtained if only internal standards, such as human lymphocytes, etc., are added to the samples, thus results in a more precise detection of aneuploidy. In 11 cases of head and neck tumors, DNA aneuploidy was observed in six out of seven cases that were histopathologically diagnosed as being malignant tumors, showing a correlation with the pathological studies as a whole. Further clinical studies may prove that the present method can be useful for the pathological, supplementary diagnostic and prognostic evaluations.
采用流式细胞术进行了研究DNA非整倍体的基础实验和临床试验。在流式细胞术检测中，发现DNA直方图中的峰值位置不稳定。然而，如果仅向样本中添加内标，如人类淋巴细胞等，就可以获得相对稳定的DNA指数，从而更精确地检测非整倍体。在11例头颈部肿瘤中，7例经组织病理学诊断为恶性肿瘤的病例中有6例观察到DNA非整倍体，总体上与病理研究相关。进一步的临床研究可能证明本方法可用于病理、辅助诊断和预后评估。
