Oh-ishi S, Tsuji N, Hayashi I
Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Jpn J Pharmacol. 1989 May;50(1):11-8. doi: 10.1254/jjp.50.11.
In order to elucidate the biological role of T-kininogen, it's levels in plasma, exudate, and liver were measured by radioimmunoassay in rats following the induction of carrageenin pleurisy. T-kininogen level in the liver microsomes was increased markedly at 8-24 hr after the carrageenin injection, and its plasma level increased at 24-48 hr with a delay. Pretreatment with dexamethasone suppressed the pleural exudate accumulation almost completely, but inhibited the T-kininogen level in plasma or liver only partially. When rats were pretreated with carrageenin injection into a paw 2 days prior to intrapleural injection of carrageenin, the pretreatment did not affect the pleurisy development, even though the plasma level of T-kininogen was greatly increased. Increased level of T-kininogen correlated well with the thiol protease inhibitor activity found in the plasma and exudate. These results indicate that an increase in the T-kininogen level does not influence the exudation, but might act as an inhibitor of thiol proteases that could be released at the inflammatory site.
为阐明T -激肽原的生物学作用，在角叉菜胶诱导大鼠胸膜炎后，采用放射免疫分析法测定其血浆、渗出液及肝脏中的水平。角叉菜胶注射后8 - 24小时，肝脏微粒体中的T -激肽原水平显著升高，血浆水平在24 - 48小时延迟升高。地塞米松预处理几乎完全抑制了胸腔渗出液的积聚，但仅部分抑制血浆或肝脏中的T -激肽原水平。当在胸腔内注射角叉菜胶前两天预先向大鼠爪部注射角叉菜胶时，尽管血浆中T -激肽原水平大幅升高，但预处理并不影响胸膜炎的发展。T -激肽原水平的升高与血浆和渗出液中发现的巯基蛋白酶抑制剂活性密切相关。这些结果表明，T -激肽原水平的升高并不影响渗出，但可能作为一种巯基蛋白酶抑制剂，抑制在炎症部位可能释放的巯基蛋白酶。
