Nerke O, Benöhr H C, Thorau U, Waller H D
Klin Wochenschr. 1978 Jan 1;56(1):43-7. doi: 10.1007/BF01476742.
Platelets of patients with thrombocytosis following splenectomy, in chronic granulocytic leukaemia and in polycythaemia vera were separated into five fractions by centrifugation in discontinuous Ficoll density gradient. Platelet volume, content of protein and enzyme activities of lactic dehydrogenase, phosphoglycerate kinase and glyceraldehyde phosphate dehydrogenase were distinctly higher for the three groups in the heavy fraction IV compared with the light fraction I. With regard to the platelet volume, however, these differences were compensated almost completely like in the normal persons.
通过在不连续的Ficoll密度梯度中离心,将脾切除术后血小板增多症患者、慢性粒细胞白血病患者和真性红细胞增多症患者的血小板分离为五个部分。与轻组分I相比,三组患者在重组分IV中的血小板体积、蛋白质含量以及乳酸脱氢酶、磷酸甘油酸激酶和甘油醛磷酸脱氢酶的酶活性明显更高。然而,就血小板体积而言,这些差异几乎完全得到了补偿,就像正常人一样。