PsbS 相互作用参与拟南芥中能量耗散的激活。
PsbS interactions involved in the activation of energy dissipation in Arabidopsis.
机构信息
Plant Biochemistry, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.
Molecular Proteomics Laboratory, Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.
出版信息
Nat Plants. 2016 Feb 1;2:15225. doi: 10.1038/nplants.2015.225.
The non-photochemical quenching of light energy as heat (NPQ) is an important photoprotective mechanism that is activated in plants when light absorption exceeds the capacity of light utilization in photosynthesis. The PsbS protein plays a central role in this process and is supposed to activate NPQ through specific, light-regulated interactions with photosystem (PS) II antenna proteins. However, NPQ-specific interaction partners of PsbS in the thylakoid membrane are still unknown. Here, we have determined the localization and protein interactions of PsbS in thylakoid membranes in the NPQ-inactive (dark) and NPQ-active (light) states. Our results corroborate a localization of PsbS in PSII supercomplexes and support the model that the light activation of NPQ is based on the monomerization of dimeric PsbS and a light-induced enhanced interaction of PsbS with Lhcb1, the major component of trimeric light-harvesting complexes in PSII.
光能的非光化学猝灭（NPQ）作为热能是一种重要的光保护机制，当植物吸收的光能超过光合作用中光能利用的能力时，这种机制就会被激活。PsbS 蛋白在这个过程中起着核心作用，它通过与光系统（PS）II 天线蛋白的特定、光调控相互作用来激活 NPQ。然而，在类囊体膜中，PsbS 的 NPQ 特异性相互作用伙伴仍然未知。在这里，我们确定了 PsbS 在 NPQ 非活跃（黑暗）和 NPQ 活跃（光照）状态下的类囊体膜中的定位和蛋白相互作用。我们的结果证实了 PsbS 在 PSII 超复合物中的定位，并支持这样的模型，即 NPQ 的光激活是基于二聚体 PsbS 的单体化和 PsbS 与 Lhcb1（PSII 中三聚体光捕获复合物的主要成分）的光诱导增强相互作用。
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