Kirby M C, Sikoryn T A, Hukins D W, Aspden R M
Department of Medical Biophysics, University of Manchester, UK.
J Biomed Eng. 1989 May;11(3):192-6. doi: 10.1016/0141-5425(89)90139-8.
Stress-strain curves were recorded from anterior and posterior longitudinal ligaments and ligamenta flava dissected from pig lumbar spines. Ligaments were examined during extension by light microscopy, to observe crimp structure, and by X-ray diffraction, to determine collagen fibril orientations. Scanning electron microscopy (SEM) was used to examine ligaments fixed at high and low strains. Initial stages of ligament extension involved alignment of collagen fibrils. Collagen fibrils in unstrained ligamentum flavum were much more disoriented than in the longitudinal ligaments. Thus, fibril alignment, and consequent stiffening, occurred at much higher strains than for longitudinal ligaments, allowing ligamentum flavum to exploit the extensibility of its elastin.
从猪腰椎中分离出前纵韧带、后纵韧带和黄韧带，并记录其应力-应变曲线。在伸展过程中，通过光学显微镜观察韧带的卷曲结构，通过X射线衍射确定胶原纤维的取向。使用扫描电子显微镜（SEM）检查在高应变和低应变下固定的韧带。韧带伸展的初始阶段涉及胶原纤维的排列。未受拉伸的黄韧带中的胶原纤维比纵韧带中的更加无序。因此，纤维排列以及随之而来的硬化发生在比纵韧带更高的应变下，使得黄韧带能够利用其弹性蛋白的可伸展性。
