Zhang H-Q, Guo X, Guo S-Q, Wang Q, Chen X-Q, Li X-N, Guo L-S
Department of General Surgery, The First Central Hospital of Baoding, Baoding, China.
Eur Rev Med Pharmacol Sci. 2016 May;20(10):2038-44.
To study the stathmin (STMN1) expression in colorectal cancer and tumor-adjacent normal tissue and discuss its prognostic significance in colon cancer.
STMN1 was tested with qRT-PCR in 30 samples of fresh colon cancer tissue and tumor-adjacent issue, and with immunohistochemical SP method in 105 samples of fresh colon cancer tissue and tumor-adjacent issue to analyze the association between its expression and clinical pathological parameters. Clinical data was combined to study the relationship between STMN1 expression and 5-year survival rate. Univariate analysis and Cox multivariate regression were performed to study the correlation between STMN1 expression and prognosis.
The mRNA and protein level of STMN1 were significantly higher in colon cancer samples than tumor-adjacent normal tissues (p<0.05). STMN1 expression was independent of patient age, gender or location, but significantly related to lymph node metastasis and TNM staging (p<0.05). Survival analysis by Kaplan-Meier method showed that STMN1 expression was significantly related with the survival of colon cancer patients. The median survival time of STMN1-positive patients (37.5 months) was significantly shorter than STMN1-negative patients (57.1 months, p<0.05). Cox multivariate regression indicated that STMN1 is independent prognostic factors predicting the development, invasion and metastasis of colon cancer (p<0.05).
STMN1 overexpression in colon cancer is independently associated with improved survival and significantly related to the development of the disease. Our findings suggest that presence of a STMN1-prognosis interaction that potentially determines clinical outcome.
研究结直肠癌及癌旁正常组织中微管相关蛋白2（STMN1）的表达情况，并探讨其在结肠癌中的预后意义。
采用qRT-PCR检测30例新鲜结肠癌组织及癌旁组织样本中的STMN1，采用免疫组织化学SP法检测105例新鲜结肠癌组织及癌旁组织样本中的STMN1，分析其表达与临床病理参数之间的相关性。结合临床资料研究STMN1表达与5年生存率之间的关系。进行单因素分析和Cox多因素回归分析以研究STMN1表达与预后的相关性。
结肠癌样本中STMN1的mRNA和蛋白水平显著高于癌旁正常组织（p<0.05）。STMN1表达与患者年龄、性别或肿瘤位置无关，但与淋巴结转移及TNM分期显著相关（p<0.05）。Kaplan-Meier法生存分析显示，STMN1表达与结肠癌患者的生存显著相关。STMN1阳性患者的中位生存时间（37.5个月）显著短于STMN1阴性患者（57.1个月，p<0.05）。Cox多因素回归分析表明，STMN1是预测结肠癌发生、侵袭和转移的独立预后因素（p<0.05）。
结肠癌中STMN1过表达与生存改善独立相关，且与疾病发展显著相关。我们的研究结果提示存在一种可能决定临床结局的STMN1-预后相互作用。
