Taracha Ewa, Kaniuga Ewelina, Wyszogrodzka Edyta, Płaźnik Adam, Stefański Roman, Chrapusta Stanisław J
Department of Neurochemistry, Institute of Psychiatry and Neurology, 9 Sobieskiego St., 02-957, Warsaw, Poland.
Department of Pharmacology and Physiology of the Nervous System, Institute of Psychiatry and Neurology, 9 Sobieskiego St., 02-957, Warsaw, Poland.
Psychopharmacology (Berl). 2016 Jul;233(14):2827-40. doi: 10.1007/s00213-016-4328-4. Epub 2016 Jun 2.
Our previous studies showed promise for using sensitization of the frequency-modulated 50-kHz vocalization response to amphetamine (AMPH) as an index of rat vulnerability to AMPH addiction.
This study aimed to test the utility of sensitizing frequency-modulated (FM) 50-kHz vocalization in the AMPH self-administration paradigm as well as the ability of N-acetylcysteine to prevent self-administration relapse.
Rats were subjected to the so-called two-injection protocol of sensitization (TIPS) using AMPH and were categorized as low-sensitized callers (LCTIPS) or high-sensitized callers (HCTIPS) based on the individual outcomes. Then, they were given 44 sessions of AMPH self-administration followed by a 17-session N-acetylcysteine-aided extinction course and a single session of AMPH-primed self-administration reinstatement.
LCTIPS compared to HCTIPS rats showed no considerable difference in the FM 50-kHz vocalization rate during the self-administration training or extinction course, but they were considerably more likely to acquire AMPH self-administration and experience drug-induced reinstatement of this trait. Moreover, the LCTIPS rats were more likely than HCTIPS rats to have a markedly higher FM 50-kHz vocalization rate after AMPH reinstatement. N-acetylcysteine did not affect the course of self-administration extinction or the instrumental or FM 50-kHz vocalization responses to AMPH reinstatement.
There is no link between the FM 50-kHz vocalization and key characteristics of AMPH self-administration. Additionally, N-acetylcysteine does not help prevent AMPH self-administration relapse. However, there is a high predictive value for poor sensitization of the FM 50-kHz vocalization response to AMPH with respect to the acquisition and maintenance of self-administration of this psychostimulant.
我们之前的研究表明，利用对苯丙胺（AMPH）的调频50千赫发声反应的敏化作用作为大鼠对AMPH成瘾易感性的指标具有前景。
本研究旨在测试在AMPH自我给药范式中调频（FM）50千赫发声的敏化作用的效用，以及N-乙酰半胱氨酸预防自我给药复发的能力。
使用AMPH对大鼠进行所谓的双注射敏化方案（TIPS），并根据个体结果将其分类为低敏化发声者（LCTIPS）或高敏化发声者（HCTIPS）。然后，让它们进行44次AMPH自我给药实验，随后进行17次N-乙酰半胱氨酸辅助消退疗程以及单次AMPH激发的自我给药复吸实验。
与HCTIPS大鼠相比，LCTIPS大鼠在自我给药训练或消退疗程期间的FM 50千赫发声率没有显著差异，但它们更有可能学会AMPH自我给药并经历药物诱导的该行为复吸。此外，LCTIPS大鼠比HCTIPS大鼠在AMPH激发后更有可能具有明显更高的FM 50千赫发声率。N-乙酰半胱氨酸不影响自我给药消退过程或对AMPH激发的工具性或FM 50千赫发声反应。
FM 50千赫发声与AMPH自我给药的关键特征之间没有关联。此外，N-乙酰半胱氨酸无助于预防AMPH自我给药复发。然而，对于这种精神兴奋剂的自我给药的获得和维持，FM 50千赫发声反应对AMPH的低敏化具有较高的预测价值。
