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[苹果螺培养神经元膜中的低阈值和高阈值钙电流]

[Low- and high-threshold calcium current in the membrane of cultured neurons of Helix pomatia].

作者信息

Lozinskaia I M, Shuba Ia M

出版信息

Neirofiziologiia. 1989;21(1):127-9.

PMID:2725779
Abstract

Two components of the inward calcium current were observed in the membrane of cultured Helix pomatia neurons by the patch clamp method in its whole cell configuration. The first one was activated at high-negative membrane potentials (-70- -60 mV), had low amplitude (200 pA at 15 mmol/l of Ca2+ ions as a charge carrier) and displayed time-dependent inactivation. A second, larger current component (2 nA, the same conditions) appeared at more positive potentials (-20 mV). Its time-dependent inactivation was much less expressed. The tail currents of the low amplitude component were slower than those associated with the high-amplitude component.

摘要

采用膜片钳全细胞记录模式,在培养的苹果蜗牛神经元膜上观察到内向钙电流的两个成分。第一个成分在高负膜电位(-70至-60mV)时被激活,幅度较低(以15mmol/l Ca2+离子作为载流子时为200pA),并表现出时间依赖性失活。第二个更大的电流成分(相同条件下为2nA)出现在更正的电位(-20mV)。其时间依赖性失活表现得要少得多。低幅度成分的尾电流比高幅度成分的尾电流慢。

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