Núñez-Acuña Gustavo, Boltaña Sebastián, Gallardo-Escárate Cristian
Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research, Department of Oceanography, Universidad de Concepción, Concepción 4030000, Chile.
Int J Mol Sci. 2016 May 31;17(6):700. doi: 10.3390/ijms17060700.
Scientific efforts to elucidate the mechanisms of chemical communication between organisms in marine environments are increasing. This study applied novel molecular technology to outline the effects of two xenobiotic drugs, deltamethrin (DM) and azamethiphos (AZA), on the neurotransmission system of the copepod ectoparasite Caligus rogercresseyi. Transcriptome sequencing and bioinformatics analyses were conducted to evaluate treatment effects on the glutamatergic synaptic pathway of the parasite, which is closely related to chemoreception and neurotransmission. After drug treatment with DM or AZA, stochastic mRNA expression patterns of glutamatergic synapse pathway components were observed. Both DM and AZA promoted a down-regulation of the glutamate-ammonia ligase, and DM activated a metabotropic glutamate receptor that is a suggested inhibitor of neurotransmission. Furthermore, the delousing drugs drove complex rearrangements in the distribution of mapped reads for specific metabotropic glutamate receptor domains. This study introduces a novel methodological approach that produces high-quality results from transcriptomic data. Using this approach, DM and AZA were found to alter the expression of numerous mRNAs tightly linked to the glutamatergic signaling pathway. These data suggest possible new targets for xenobiotic drugs that play key roles in the delousing effects of antiparasitics in sea lice.
阐明海洋环境中生物间化学通讯机制的科学研究正在不断增加。本研究应用新型分子技术概述了两种外源性药物溴氰菊酯（DM）和啶虫脒（AZA）对桡足类体外寄生虫罗氏海盘虫神经传递系统的影响。进行了转录组测序和生物信息学分析，以评估药物处理对与化学感受和神经传递密切相关的寄生虫谷氨酸能突触通路的影响。在用DM或AZA进行药物处理后，观察到谷氨酸能突触通路成分的随机mRNA表达模式。DM和AZA均促进了谷氨酸-氨连接酶的下调，并且DM激活了一种代谢型谷氨酸受体，该受体被认为是神经传递的抑制剂。此外，杀虱药物促使特定代谢型谷氨酸受体结构域的映射读数分布发生复杂重排。本研究引入了一种新的方法学途径，可从转录组数据中产生高质量结果。使用这种方法，发现DM和AZA改变了与谷氨酸能信号通路紧密相关的众多mRNA的表达。这些数据表明外源性药物可能有新的靶点，这些靶点在抗寄生虫剂对海虱的杀虱作用中起关键作用。
