Lai M E, Farci P, Figus A, Mulargia A P, Orgiana G, Cuboni C, Angioni G, Balestrieri A
Università degli Studi di Cagliari, Istituto di Medicina Interna.
Minerva Med. 1989 Apr;80(4):335-9.
Hepatitis B virus (HBV) DNA sequences were assessed in 26 patients with acute type B hepatitis, using dot-blot hybridization technique from peripheral blood mononuclear cells (PBMC), during different phases of the illness. At clinical presentation, 15% of patients showed HBV-DNA sequences in PBMC, while serum HBV-DNA was detected in 58% of patients. During clinical improvement 50% of patients had HBV-DNA in PBMC but only 11.5% were positive for serum HBV-DNA. Twenty-three (88.5%) patients recovered and cleared HBV-DNA from serum and from PBMC; three (11.5%) patients with acute hepatitis progressing to chronicity showed persistently HBV-DNA sequences in serum and in PBMC. In conclusion, our study shows that HBV-DNA sequences may be found in PBMC, transiently in patients with acute hepatitis followed by recovery, persistently in patients with acute hepatitis progressing to chronicity.
采用斑点杂交技术，对26例急性乙型肝炎患者在疾病不同阶段的外周血单个核细胞（PBMC）中的乙型肝炎病毒（HBV）DNA序列进行了评估。在临床表现期，15%的患者PBMC中显示有HBV - DNA序列，而58%的患者血清中检测到HBV - DNA。在临床症状改善期，50%的患者PBMC中有HBV - DNA，但血清HBV - DNA阳性的患者仅占11.5%。23例（88.5%）患者康复，血清和PBMC中的HBV - DNA均清除；3例（11.5%）急性肝炎进展为慢性肝炎的患者血清和PBMC中持续显示有HBV - DNA序列。总之，我们的研究表明，急性肝炎恢复患者的PBMC中可短暂检测到HBV - DNA序列，而急性肝炎进展为慢性肝炎的患者PBMC中则持续存在该序列。
