Regional distribution of gamma-aminobutyrate and L-glutamate uptake into synaptic vesicles isolated from rat brain.

The ATP-dependent uptake of GABA and L-glutamate into synaptic vesicles isolated from 4 different regions of the rat brain was studied. The regional distribution of the vesicular uptake was related to the Na+-dependent synaptosomal uptake, which, as a first approximation, corresponds to the distribution of GABAergic and glutamatergic terminals. The ratio found between the vesicular GABA and L-glutamate uptake varied between 1.3 and 6.2. This indicates that GABA and L-glutamate are taken up into different vesicle populations.