使用mRNA分析进行体液鉴定。
Body Fluid Identification Using mRNA Profiling.
作者信息
Roeder Amy D, Haas Cordula
机构信息
Cellmark, Blacklands Way, Abingdon, Oxfordshire, OX14 1DY, UK.
Institute of Legal Medicine, University of Zurich, Zürich, Switzerland.
出版信息
Methods Mol Biol. 2016;1420:13-31. doi: 10.1007/978-1-4939-3597-0_2.
RNA analysis is a valuable tool for the identification of the forensically relevant body fluids, saliva, blood, menstrual blood, cervicovaginal fluid, and semen. Multiple human mRNA and bacterial RNA markers have been identified for each of these body fluids. RNA and DNA can be coextracted from the same portion of a sample and RNA markers for different body fluids can be multiplexed in a single PCR, thereby maximizing the number of analyses that can be performed with limited sample material.
RNA分析是鉴定法医相关体液（唾液、血液、经血、宫颈阴道液和精液）的宝贵工具。已针对每种体液鉴定出多种人类mRNA和细菌RNA标记物。RNA和DNA可以从样品的同一部分中共提取，并且不同体液的RNA标记物可以在单个PCR中进行多重分析，从而在有限的样品材料条件下最大限度地增加可进行的分析数量。
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