用于阴道分泌物和月经血鉴定的mRNA分析
mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification.
作者信息
Jakubowska Joanna, Maciejewska Agnieszka, Pawłowski Ryszard
机构信息
Department of Forensic Medicine, Laboratory of Forensic Biology and Genetics, Medical University of Gdansk, ul.Dębowa 23, Gdańsk, 80-204, Poland.
出版信息
Methods Mol Biol. 2016;1420:33-42. doi: 10.1007/978-1-4939-3597-0_3.
Recently mRNA profiling has been widely proposed as a universal tool for biological fluids identification. Here, we describe a test for vaginal fluid identification that combines detection of five markers: vaginal mRNAs and Lactobacilli in end-point PCR reaction. The test detects the following transcripts: HBD1 (Human beta-defensin 1), MUC4 (Mucin 4), MMP11 (Matrix metalloproteinase 11), housekeeping gene G6PDH (glucose 6-phosphate dehydrogenase), and the 16S-23S rRNA intergenic spacer regions of L. crispatus and L. gasseri/L. johnsonii. Simultaneous analysis of five vaginal markers and a housekeeping gene ensures high specificity and reliability in the detection of vaginal material, which could not be obtained using detection of a single marker.
最近，mRNA 分析已被广泛提议作为生物体液鉴定的通用工具。在此，我们描述了一种用于阴道液鉴定的测试方法，该方法在终点 PCR 反应中结合了五种标志物的检测：阴道 mRNA 和乳酸杆菌。该测试可检测以下转录本：HBD1（人β-防御素 1）、MUC4（粘蛋白 4）、MMP11（基质金属蛋白酶 11）、管家基因 G6PDH（葡萄糖 6-磷酸脱氢酶），以及卷曲乳杆菌和加氏乳杆菌/约氏乳杆菌的 16S - 23S rRNA 基因间隔区。同时分析五种阴道标志物和一个管家基因可确保在检测阴道物质时具有高特异性和可靠性，而使用单一标志物检测则无法获得这种效果。
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