HIrisPlex系统实用指南：从DNA同时预测眼睛和头发颜色
A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA.
作者信息
Walsh Susan, Kayser Manfred
机构信息
Department of Biology, Indiana University-Purdue University Indianapolis (IUPUI), 723 W. Michigan St., SL 350, Indianapolis, IN, 46202, USA.
Department of Forensic Molecular Biology, Erasmus MC University Medical Center Rotterdam, 3000 CB, Rotterdam, The Netherlands.
出版信息
Methods Mol Biol. 2016;1420:213-31. doi: 10.1007/978-1-4939-3597-0_17.
The HIrisPlex system, which consists of two parts, allows the simultaneous prediction of eye and hair color from DNA, e.g., samples extracted from crime scene evidence. The first part is a highly sensitive multiplex genotyping assay consisting of 24 DNA markers using SNaPshot™ chemistry, for analysis on all Capillary Electrophoresis machines. The second part consists of statistical models that respectively establish eye and hair color prediction probabilities from complete and incomplete genotype profiles using parameters generated from large genotype and phenotype databases. This combined prediction tool constitutes the online system freely available to users. Here we provide a practical guide on how to use the HIrisPlex system for forensic and other DNA applications.
HIrisPlex系统由两部分组成，可根据DNA（例如从犯罪现场证据中提取的样本）同时预测眼睛和头发颜色。第一部分是一种高度灵敏的多重基因分型检测方法，使用SNaPshot™化学技术，由24个DNA标记组成，可在所有毛细管电泳仪上进行分析。第二部分由统计模型组成，这些模型利用从大型基因型和表型数据库生成的参数，分别根据完整和不完整的基因型谱建立眼睛和头发颜色的预测概率。这个组合式预测工具构成了可供用户免费使用的在线系统。在此，我们提供一份关于如何将HIrisPlex系统用于法医及其他DNA应用的实用指南。
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