Li Lin, Wu Xiaodong, Liu Fuxiao, Wang Zhiliang, Liu Chunju, Wang Qinghua, Bao Jingyue
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
J Virol Methods. 2016 Sep;235:131-133. doi: 10.1016/j.jviromet.2016.05.019. Epub 2016 Jun 1.
Peste des petits ruminants virus (PPRV) is the cause agent of peste des petitis ruminants (PPR). A novel lineage IV PPRV has reemerged in China in 2013 and 2014. Mass vaccination was implemented in most provinces in China. In order to detect lineage IV PPRV in clinical samples and to distinguish rapidly it from the other lineages PPRVs, a real-time RT-PCR assay was developed. This assay showed high sensitivity, specificity and efficiency in differentiating the lineage IV PPRV from others. The performance of this assay was evaluated by positive clinical samples of lineage IV viruses. This new real-time RT-PCR assay will facilitate epidemiological investigations and rapid differentiatial diagnosis in areas where lineage IV viruses are circulating.
小反刍兽疫病毒（PPRV）是小反刍兽疫（PPR）的病原。一种新型IV型PPRV于2013年和2014年在中国再次出现。中国大多数省份都实施了大规模疫苗接种。为了检测临床样本中的IV型PPRV并将其与其他型PPRV快速区分开来，开发了一种实时RT-PCR检测方法。该检测方法在区分IV型PPRV与其他型PPRV时显示出高灵敏度、特异性和效率。通过IV型病毒的阳性临床样本对该检测方法的性能进行了评估。这种新的实时RT-PCR检测方法将有助于在IV型病毒流行地区进行流行病学调查和快速鉴别诊断。
