Chen Ren-zhi, Zhu Min, Ji Yu-lin
Sichuan Da Xue Xue Bao Yi Xue Ban. 2016 Mar;47(2):222-6.
To explore the comorbidity mechanism of asthma and depression.
A self-developed questionnaire, which also contained Asthma Control Test (ACT), Asthma Quality of Life Questionnaire (AQLQ) and Hamilton Rating Scale for depression ( HRSD), was administered in 41 participants with asthma (AS), asthma and depression (AD), or none any of these conditions (health control, HC). Lung function and blood levels of eosnophils and IgE were also detected in those in the AS and AD groups. Blood CD4+ T cells were isolated in all of the participants to extract RNA for reverse transcription to cDNA. Real-time polymerase chain reaction (RT-PCR) was performed using cDNA as a template to detected the expression levels of HTR1A, HTR2A, HTR7, SLC6A4, and B2M genes.
Participants with AS and AD had lower expression level of SLC6A4 than the healthy controls (P = 0.000). The expression level of HTR2A in participants with AS was lower than that in the healthy controls (P = 0.021) and marginally lower than that in participants with AD (P = 0.077). Participants with AD had lower AQLQ scores than participants with AS (P = 0.004).
Asthma and depression is correlated at gene level. Decreased expression of SLC6A4 gene may be one of the possible comorbidity mechanisms of asthma and depression.
探讨哮喘与抑郁症的共病机制。
对41名患有哮喘（AS）、哮喘合并抑郁症（AD）或无上述任何疾病（健康对照，HC）的参与者进行了一份自行编制的问卷调查，该问卷还包含哮喘控制测试（ACT）、哮喘生活质量问卷（AQLQ）和汉密尔顿抑郁量表（HRSD）。同时检测了AS组和AD组参与者的肺功能以及嗜酸性粒细胞和IgE的血液水平。分离了所有参与者的血液CD4+ T细胞，提取RNA并逆转录为cDNA。以cDNA为模板进行实时聚合酶链反应（RT-PCR），以检测HTR1A、HTR2A、HTR7、SLC6A4和B2M基因的表达水平。
AS组和AD组参与者的SLC6A4表达水平低于健康对照组（P = 0.000）。AS组参与者的HTR2A表达水平低于健康对照组（P = 0.021），略低于AD组参与者（P = 0.077）。AD组参与者的AQLQ得分低于AS组参与者（P = 0.004）。
哮喘与抑郁症在基因水平上存在关联。SLC6A4基因表达降低可能是哮喘与抑郁症共病的可能机制之一。
