斑马鱼的胚胎细胞培养
Embryonic cell culture in zebrafish.
作者信息
Ciarlo C A, Zon L I
机构信息
Harvard Medical School and Children's Hospital, Boston, MA, United States.
Children's Hospital and Dana Farber Cancer Institute, Boston, MA, United States; Harvard University, Cambridge, MA, United States.
出版信息
Methods Cell Biol. 2016;133:1-10. doi: 10.1016/bs.mcb.2016.02.010. Epub 2016 Mar 31.
Zebrafish embryonic cell cultures have many useful properties that make them complementary to intact embryos for a wide range of studies. Embryonic cell cultures allow for maintenance of transient cell populations, control of chemical and mechanical cues received by cells, and facile chemical screening. Zebrafish cells can be cultured in either heterogeneous or homogeneous cultures from a wide range of developmental time points. Here we describe two methods with particular applicability to chemical screening: a method for the culture of blastomeres for directed differentiation toward the myogenic lineage and a method for the culture of neural crest cells in heterogeneous cultures from early somitogenesis embryos.
斑马鱼胚胎细胞培养具有许多有用的特性，使其在广泛的研究中成为完整胚胎的补充。胚胎细胞培养能够维持短暂的细胞群体，控制细胞所接收的化学和机械信号，并便于进行化学筛选。斑马鱼细胞可以从广泛的发育时间点进行异质或均质培养。在这里，我们描述两种特别适用于化学筛选的方法：一种用于培养卵裂球以定向分化为肌源性谱系的方法，以及一种用于从早期体节形成胚胎的异质培养物中培养神经嵴细胞的方法。
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