Sauer M, Dörge A, Thurau K, Beck F X
Physiologisches Institut, Universität München, Federal Republic of Germany.
Pflugers Arch. 1989 Apr;413(6):651-5. doi: 10.1007/BF00581816.
Sodium, phosphorus, chloride and potassium concentrations were measured by a new method in individual principal and intercalated cells in the cortical collecting duct in vitro. Electron microprobe analysis was applied to freeze-dried cryosections of the isolated perfused rabbit cortical collecting duct. Cell analyses were performed under control conditions and after addition of ouabain to the bath. Under control conditions similar sodium, potassium, chloride, and phosphorus concentration (means +/- SEM) were observed in principal (10.0 +/- 0.6, 126.5 +/- 2.7, 24.6 +/- 1.0, and 121.5 +/- 3.5 mmol/kg wet weight, respectively) and intercalated cells (9.0 +/- 0.9, 127.1 +/- 4.2, 27.4 +/- 1.8, and 118.7 +/- 4.9 mmol/kg wet weight, respectively). In principal cells ouabain (10 min) caused an increase in sodium and chloride concentrations by 104 and 13 mmol/kg wet weight, and a decrease in potassium and phosphorus concentrations by 106 and 32 mmol/kg wet weight. These changes in cell element concentrations can be ascribed to an exchange of intracellular potassium against extracellular sodium and to cell swelling due to influx of extracellular fluid. The effects of ouabain on intercalated cells were far less pronounced than on principal cells. This different susceptibility to ouabain of principal and intercalated cells can be ascribed to differences in active and passive transmembrane ion transport pathways.
采用一种新方法在体外对皮质集合管中的单个主细胞和闰细胞进行钠、磷、氯和钾浓度的测量。电子微探针分析应用于分离灌注的兔皮质集合管的冷冻干燥冰冻切片。在对照条件下以及向浴液中添加哇巴因后进行细胞分析。在对照条件下，主细胞（分别为10.0±0.6、126.5±2.7、24.6±1.0和121.5±3.5 mmol/kg湿重）和闰细胞（分别为9.0±0.9、127.1±4.2、27.4±1.8和118.7±4.9 mmol/kg湿重）中观察到相似的钠、钾、氯和磷浓度。在主细胞中，哇巴因（10分钟）导致钠和氯浓度分别增加104和13 mmol/kg湿重，钾和磷浓度分别降低106和32 mmol/kg湿重。细胞元素浓度的这些变化可归因于细胞内钾与细胞外钠的交换以及由于细胞外液流入导致的细胞肿胀。哇巴因对闰细胞的影响远不如对主细胞明显。主细胞和闰细胞对哇巴因的这种不同敏感性可归因于主动和被动跨膜离子转运途径的差异。
