静脉注射免疫球蛋白替代治疗不会改变常见可变免疫缺陷患者的多形核白细胞功能和表面受体表达。
Intravenous immunoglobulin replacement treatment does not alter polymorphonuclear leukocytes function and surface receptors expression in patients with common variable immunodeficiency.
作者信息
Prezzo Alessandro, Cavaliere Filomena Monica, Bilotta Caterina, Iacobini Metello, Quinti Isabella
机构信息
Department of Molecular Medicine, Sapienza University of Rome, Italy.
Department of Paediatrics, Sapienza University of Rome, Italy.
出版信息
Cell Immunol. 2016 Aug-Sep;306-307:25-34. doi: 10.1016/j.cellimm.2016.05.006. Epub 2016 May 28.
The study of the expression of CD16, CD11b and Siglec 9 receptors and the oxidative burst provides insights on polymorphonuclear leukocytes (PMN) functionality in common variable immunodeficiency (CVID) and on the possible effects of intravenous immunoglobulin (IVIg) infusion. We evaluated in vivo before and soon after IVIg administration the CD16, CD11b and Siglec 9 expression on unstimulated and Escherichia coli-stimulated PMN and the oxidative burst induced by Escherichia coli and PMA. The E. coli stimulation up-regulated CD16 and Siglec 9 expression and it induced a strong CD11b up-regulation at baseline and soon after IVIg. The oxidative burst overlapped that observed in healthy donors when induced by Escherichia coli while it increased when induced by PMA. Soon after IVIg infusion, the oxidative burst decreased only when induced by PMA. Our results showed that the IVIg infusion in vivo had a minimal effect on CVID's PMN.
对CD16、CD11b和唾液酸结合免疫球蛋白样凝集素9（Siglec 9）受体表达及氧化爆发的研究，为常见可变免疫缺陷（CVID）中多形核白细胞（PMN）的功能以及静脉注射免疫球蛋白（IVIg）输注的可能影响提供了见解。我们在IVIg给药前及给药后不久，评估了未刺激和经大肠杆菌刺激的PMN上CD16、CD11b和Siglec 9的表达，以及大肠杆菌和佛波酯（PMA）诱导的氧化爆发。大肠杆菌刺激上调了CD16和Siglec 9的表达，并在基线时及IVIg给药后不久诱导了强烈的CD11b上调。当由大肠杆菌诱导时，氧化爆发与健康供体中观察到的情况重叠，而当由PMA诱导时氧化爆发增加。IVIg输注后不久，仅在由PMA诱导时氧化爆发减少。我们的结果表明，体内IVIg输注对CVID患者的PMN影响极小。
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