腹泻型肠易激综合征和抑郁症患者粪便微生物群特征相似。

Similar Fecal Microbiota Signatures in Patients With Diarrhea-Predominant Irritable Bowel Syndrome and Patients With Depression.

机构信息

Department of Gastroenterology, Peking University Third Hospital, Beijing, China.

Department of Biomedical Engineering, Center for Quantitative Biology, College of Engineering, Peking University, Beijing, China.

出版信息

Clin Gastroenterol Hepatol. 2016 Nov;14(11):1602-1611.e5. doi: 10.1016/j.cgh.2016.05.033. Epub 2016 Jun 4.

DOI:10.1016/j.cgh.2016.05.033

PMID:27266978

Abstract

BACKGROUND & AIMS: Patients with irritable bowel syndrome (IBS) often have psychiatric comorbidities. Alterations in the intestinal microbiota have been associated with IBS and depression, but it is not clear if there is a microbial relationship between these disorders. We studied the profiles of fecal microbiota samples from patients with IBS, depression, or comorbidities of IBS and depression; we determined the relationships among these profiles and clinical and pathophysiological features of these disorders.

METHODS

We used 454 pyrosequencing to analyze fecal microbiota samples from 100 subjects (40 with diarrhea-predominant IBS [IBS-D], 15 with depression, 25 with comorbidities of IBS and depression, and 20 healthy individuals [controls]), recruited at Peking University. Abdominal and psychological symptoms were evaluated with validated questionnaires. Visceral sensitivity was evaluated using a barostat. Colonic mucosal inflammation was assayed by immunohistochemical analyses of sigmoid tissue biopsy specimens.

RESULTS

Fecal microbiota signatures were similar between patients with IBS-D and depression in that they were less diverse than samples from controls and had similar abundances of alterations. They were characterized by high proportions of Bacteroides (type I), Prevotella (type II), or nondominant microbiota (type III). Most patients with IBS-D or depression had type I or type II profiles (IBS-D had 85% type I and type II profiles, depression had 80% type I and type II profiles). Colon tissues from patients with type I or type II profiles had higher levels of inflammatory markers than colon tissues from patients with type III profiles. The level of colon inflammation correlated with the severity of IBS symptoms.

CONCLUSIONS

Patients with IBS-D and depression have similar alterations in fecal microbiota; these might be related to the pathogenesis of these disorders. We identified 3 microbial profiles in patients that could indicate different subtypes of IBS and depression or be used as diagnostic biomarkers.

摘要

背景与目的

肠易激综合征（IBS）患者常伴有精神共病。肠道微生物群的改变与 IBS 和抑郁症有关，但这些疾病之间是否存在微生物关系尚不清楚。我们研究了 IBS、抑郁症或 IBS 和抑郁症共病患者的粪便微生物群样本的特征；我们确定了这些特征之间的关系以及这些疾病的临床和病理生理学特征。

方法

我们使用 454 焦磷酸测序分析了 100 名受试者（40 名腹泻为主的 IBS [IBS-D]、15 名抑郁症、25 名 IBS 和抑郁症共病、20 名健康对照）的粪便微生物群样本，这些受试者均来自北京大学。采用经过验证的问卷评估腹部和心理症状。使用测压计评估内脏敏感性。通过对乙状结肠组织活检标本进行免疫组织化学分析来检测结肠黏膜炎症。

结果

IBS-D 患者和抑郁症患者的粪便微生物群特征相似，它们的多样性低于对照组，且存在相似数量的改变。它们的特征是高比例的拟杆菌（I 型）、普雷沃氏菌（II 型）或非优势菌群（III 型）。大多数 IBS-D 或抑郁症患者具有 I 型或 II 型特征（IBS-D 中有 85%的 I 型和 II 型特征，抑郁症中有 80%的 I 型和 II 型特征）。具有 I 型或 II 型特征的患者的结肠组织中的炎症标志物水平高于具有 III 型特征的患者。结肠炎症的程度与 IBS 症状的严重程度相关。