Tobe M, Naito K, Kurokawa Y
Division of Toxicology, National Institute of Hygienic Sciences, Tokyo, Japan.
Toxicology. 1989 May 31;56(1):79-86. doi: 10.1016/0300-483x(89)90213-8.
Groups of 20 male and 20 female Wistar rats were given formaldehyde solution in their drinking water at concentrations of 0.50, 0.10, 0.02 and 0% for 24 months. Significant decreases in body weight and food and water intake were observed in the 0.50% group of both sexes and all rats in this group died by 24 months. Various non-neoplastic lesions were observed in rats, mostly in the 0.50% group. In this group, erosions and/or ulcers were evident in both the forestomach and glandular stomach. In the forestomach, squamous cell hyperplasia with or without hyperkeratosis and downward growth of basal cells were observed. Glandular hyperplasia of the fundic mucosa was noted along the limiting ridge. A few of such changes of the upper GI tract were seen in the 0.10% group. No toxicological abnormalities were found in 0.02% group of both sexes. There were no significant differences in the incidences of any tumors among groups of both sexes. Based on these findings, the no observable effect level of formaldehyde was 0.02% in the drinking water (10 mg/kg body wt./day).
将20只雄性和20只雌性Wistar大鼠分为几组，分别给予浓度为0.50%、0.10%、0.02%和0%的甲醛溶液，持续24个月。在0.50%组的雌雄大鼠中均观察到体重、食物和水摄入量显著下降，该组所有大鼠在24个月时均死亡。在大鼠中观察到各种非肿瘤性病变，主要出现在0.50%组。在该组中，前胃和腺胃均出现糜烂和/或溃疡。在前胃中，观察到鳞状细胞增生，伴有或不伴有角化过度以及基底细胞向下生长。沿界限嵴可见胃底黏膜的腺性增生。在0.10%组中也观察到少数此类上消化道变化。在0.02%组的雌雄大鼠中均未发现毒理学异常。两组中任何肿瘤的发生率均无显著差异。基于这些发现，饮用水中甲醛的无可见效应水平为0.02%（10毫克/千克体重/天）。
