Kuan Chee Sian, Ismail Rokiah, Kwan Zhenli, Yew Su Mei, Yeo Siok Koon, Chan Chai Ling, Toh Yue Fen, Na Shiang Ling, Lee Kok Wei, Hoh Chee-Choong, Yee Wai-Yan, Ng Kee Peng
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Division of Dermatology, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
PLoS One. 2016 Jun 9;11(6):e0156119. doi: 10.1371/journal.pone.0156119. eCollection 2016.
A yeast-like organism was isolated from the skin scraping sample of a stasis dermatitis patient in the Mycology Unit Department of Medical Microbiology, University Malaya Medical Centre (UMMC), Kuala Lumpur, Malaysia. The isolate produced no pigment and was not identifiable using chromogenic agar and API 20C AUX. The fungus was identified as Metschnikowia sp. strain UM 1034, which is close to that of Metschnikowia drosophilae based on ITS- and D1/D2 domain-based phylogenetic analysis. However, the physiology of the strain was not associated to M. drosophilae. This pathogen exhibited low sensitivity to all tested azoles, echinocandins, 5-flucytosine and amphotericin B. This study provided insight into Metschnikowia sp. strain UM 1034 phenotype profiles using a Biolog phenotypic microarray (PM). The isolate utilized 373 nutrients of 760 nutrient sources and could adapt to a broad range of osmotic and pH environments. To our knowledge, this is the first report of the isolation of Metschnikowia non-pulcherrima sp. from skin scraping, revealing this rare yeast species as a potential human pathogen that may be misidentified as Candida sp. using conventional methods. Metschnikowia sp. strain UM 1034 can survive in flexible and diverse environments with a generalist lifestyle.
从马来西亚吉隆坡马来亚大学医学中心（UMMC）医学微生物学真菌科的一名淤积性皮炎患者的皮肤刮屑样本中分离出一种酵母样生物。该分离株不产生色素，使用显色琼脂和API 20C AUX无法鉴定。基于ITS和D1/D2结构域的系统发育分析，该真菌被鉴定为梅奇酵母属菌株UM 1034，与果蝇梅奇酵母相近。然而，该菌株的生理学特性与果蝇梅奇酵母不同。该病原体对所有测试的唑类、棘白菌素、5-氟胞嘧啶和两性霉素B均表现出低敏感性。本研究使用Biolog表型微阵列（PM）对梅奇酵母属菌株UM 1034的表型特征进行了深入研究。该分离株利用了760种营养源中的373种营养物质，能够适应广泛的渗透压和pH环境。据我们所知，这是首次从皮肤刮屑中分离出非美丽梅奇酵母的报告，揭示了这种罕见的酵母物种是一种潜在的人类病原体，使用传统方法可能会被误鉴定为念珠菌属。梅奇酵母属菌株UM 1034可以以一种通才的生活方式在灵活多样的环境中生存。
