Schuurmans A L, Bolt J, Mulder E
Department of Biochemistry, Erasmus University Rotterdam, The Netherlands.
Urol Int. 1989;44(2):71-6. doi: 10.1159/000281473.
Human prostate tumor cells, LNCaP, contain androgen receptors and respond to androgens with increased growth rate. Although other steroid hormone receptors are not detectable in LNCaP cells, progesterone and estradiol stimulate the growth of these cells. The androgen receptor shows considerable cross binding activity with progesterone and estradiol but not with the glucocorticoid triamcinolone acetonide. The latter steroid does not have any effect on cell proliferation. LNCaP cells respond to epidermal growth factor (EGF) with an increased growth rate. Steroids that stimulate LNCaP cell proliferation also increase the number of EGF receptors per cell. In conclusion, LNCaP cells are sensitive to EGF. Different steroids bind to the androgen receptor and stimulate the proliferation of human prostate tumor cells. This stimulation of growth is preceded by an increase in EGF receptor number per cell.
人前列腺肿瘤细胞LNCaP含有雄激素受体，并对雄激素作出反应，生长速率加快。虽然在LNCaP细胞中检测不到其他类固醇激素受体，但孕酮和雌二醇可刺激这些细胞的生长。雄激素受体与孕酮和雌二醇表现出相当大的交叉结合活性，但与糖皮质激素曲安奈德没有交叉结合活性。后一种类固醇对细胞增殖没有任何影响。LNCaP细胞对表皮生长因子（EGF）作出反应，生长速率加快。刺激LNCaP细胞增殖的类固醇也会增加每个细胞的EGF受体数量。总之，LNCaP细胞对EGF敏感。不同的类固醇与雄激素受体结合并刺激人前列腺肿瘤细胞的增殖。这种生长刺激之前是每个细胞的EGF受体数量增加。
