Aragão-Silva C W, Andrade M S, Ardisson-Araújo D M P, Fernandes J E A, Morgado F S, Báo S N, Moraes R H P, Wolff J L C, Melo F L, Ribeiro B M
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
Departamento de Entomologia, Instituto Butantan, Av. Vital Brasil, 1500, São Paulo, Brazil.
Sci Rep. 2016 Jun 10;6:23127. doi: 10.1038/srep23127.
Lonomia obliqua (Lepidoptera: Saturniidae) is a species of medical importance due to the severity of reactions caused by accidental contact with the caterpillar bristles. Several natural pathogens have been identified in L. obliqua, and among them the baculovirus Lonomia obliqua multiple nucleopolyhedrovirus (LoobMNPV). The complete genome of LoobMNPV was sequenced and shown to have 120,022 bp long with 134 putative open reading frames (ORFs). Phylogenetic analysis of the LoobMNPV genome showed that it belongs to Alphabaculovirus group I (lepidopteran-infective NPV). A total of 12 unique ORFs were identified with no homologs in other sequenced baculovirus genomes. One of these, the predicted protein encoded by loob035, showed significant identity to an eukaryotic transcription terminator factor (TTF2) from the Lepidoptera Danaus plexippus, suggesting an independent acquisition through horizontal gene transfer. Homologs of cathepsin and chitinase genes, which are involved in host integument liquefaction and viral spread, were not found in this genome. As L. obliqua presents a gregarious behavior during the larvae stage the impact of this deletion might be neglectable.
斜纹天蛾（鳞翅目：天蚕蛾科）是一种具有医学重要性的物种，因为意外接触其毛虫刚毛会引发严重反应。在斜纹天蛾中已鉴定出几种天然病原体，其中包括杆状病毒斜纹天蛾多核多角体病毒（LoobMNPV）。对LoobMNPV的全基因组进行了测序，结果显示其长度为120,022 bp，具有134个推定的开放阅读框（ORF）。对LoobMNPV基因组的系统发育分析表明，它属于甲型杆状病毒I组（感染鳞翅目的NPV）。共鉴定出12个独特的ORF，在其他已测序的杆状病毒基因组中没有同源物。其中之一，由loob035编码的预测蛋白与鳞翅目黑脉金斑蝶的真核转录终止因子（TTF2）具有显著的同源性，这表明它是通过水平基因转移独立获得的。在该基因组中未发现参与宿主表皮液化和病毒传播的组织蛋白酶和几丁质酶基因的同源物。由于斜纹天蛾在幼虫阶段表现出群居行为，这种缺失的影响可能微不足道。
