Rohrmann George F, Erlandson Martin A, Theilmann David A
Department of Microbiology, Oregon State University, Corvallis, OR, 97331-3804, USA,
Virus Genes. 2013 Oct;47(2):357-64. doi: 10.1007/s11262-013-0951-x. Epub 2013 Jul 13.
The genome sequence of a baculovirus from Hemileuca sp. was determined. The genome is 140,633 kb, has a G+C content of 38.1 %, and encodes 137 putative open-reading frames over 50 amino acids. 126 of these ORFs showed similarity to other baculovirus genes in the database including all 37 core genes. Of the remaining 11 predicted genes, one is related to a lepidopteran serpin gene. This is the first report of a baculovirus encoding a member of this family of serine protease inhibitors, and to our knowledge the first report of a viral serpin outside the Poxviridae. The genome also contained three homologous repeat sequences. Phylogenetic analysis indicated that the virus is a group II Alphabaculovirus and belongs to a lineage that includes Orgyia leucostigma, Ectropis obliqua, Apocheima cinerarium, and Euproctis pseudoconspersa nucleopolyhedroviruses.
测定了一种来自Hemileuca sp.的杆状病毒的基因组序列。该基因组大小为140,633 kb，G+C含量为38.1%，编码137个推定的、超过50个氨基酸的开放阅读框。其中126个开放阅读框与数据库中其他杆状病毒基因具有相似性，包括所有37个核心基因。在其余11个预测基因中，有一个与鳞翅目丝氨酸蛋白酶抑制剂基因相关。这是关于杆状病毒编码该丝氨酸蛋白酶抑制剂家族成员的首次报道，据我们所知，也是痘病毒科以外病毒丝氨酸蛋白酶抑制剂的首次报道。该基因组还包含三个同源重复序列。系统发育分析表明，该病毒是一种II组甲型杆状病毒，属于一个包括白纹毒蛾、斜纹夜蛾、樟蚕和茶毛虫核多角体病毒的谱系。
