Rhoads M L, Murrell K D, Cross J H, Fan P C
U.S. Department of Agriculture, Beltsville Agricultural Research Center, MD 20705.
Vet Parasitol. 1989 Mar;30(4):279-85. doi: 10.1016/0304-4017(89)90097-6.
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibody in pigs infected with a possibly new species of Taenia isolated in Taiwan is described. The test antigen ThFAS was fractionated from the cyst fluid of a heterologous cestode Taenia hydatigena. In lightly infected pigs (less than or equal to 4 recovered cysts at necropsy 17 weeks post-inoculation), antibody was detected as early as 3 weeks post-inoculation. In more heavily infected pigs (6-72 recovered cysts at necropsy 32 weeks post-inoculation), antibody was still detectable at the time of necropsy. Cysticerci were found only in the livers of the infected pigs. This ELISA should be highly useful for detecting infection of pigs with this larval cestode in regions where the presence of Taenia solium is unlikely.
本文描述了一种酶联免疫吸附测定（ELISA）法，用于检测台湾地区分离出的一种可能为新型猪带绦虫感染猪体内的抗体。检测抗原ThFAS是从异源绦虫——有钩绦虫的囊液中分离出来的。在轻度感染的猪中（接种后17周尸检时回收的囊肿少于或等于4个），接种后3周即可检测到抗体。在感染更严重的猪中（接种后32周尸检时回收6 - 72个囊肿），尸检时仍可检测到抗体。仅在感染猪的肝脏中发现囊尾蚴。在不太可能存在猪带绦虫的地区，这种ELISA法对于检测猪感染这种幼虫绦虫应非常有用。
