使用基于肽的近红外荧光探针进行体内肿瘤血管生成成像
In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes.
作者信息
Huang Rui, Conti Peter S, Chen Kai
机构信息
Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, 2250 Alcazar Street, CSC 103, Los Angeles, CA, 90033-9061, USA.
出版信息
Methods Mol Biol. 2016;1444:73-84. doi: 10.1007/978-1-4939-3721-9_8.
Near-infrared fluorescence (NIRF) imaging is an emerging imaging technique for studying diseases at the molecular level. Optical imaging with a near-infrared emitting fluorophore for targeting tumor angiogenesis offers a noninvasive method for early tumor detection and efficient monitoring of tumor response to anti-angiogenesis therapy. CD13 receptor, a zinc-dependent membrane-bound ectopeptidase, plays important roles in regulating tumor angiogenesis and the growth of new blood vessels. In this chapter, we use CD13 receptor as an example to demonstrate how to construct CD13-specific NGR-containing peptides via bioorthogonal click chemistry for visualizing and quantifying the CD13 receptor expression in vivo by means of NIRF optical imaging.
近红外荧光（NIRF）成像是一种用于在分子水平研究疾病的新兴成像技术。使用近红外发光荧光团进行光学成像以靶向肿瘤血管生成，为早期肿瘤检测和有效监测肿瘤对抗血管生成治疗的反应提供了一种非侵入性方法。CD13受体是一种锌依赖性膜结合外肽酶，在调节肿瘤血管生成和新血管生长中起重要作用。在本章中，我们以CD13受体为例，展示如何通过生物正交点击化学构建含CD13特异性NGR的肽，以通过NIRF光学成像在体内可视化和定量CD13受体表达。
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