Malide Daniela
Light Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institute of Health, Building 10, Room 6 N-309, 10 Center Drive, Bethesda, MD, 20892, USA.
Methods Mol Biol. 2016;1444:109-22. doi: 10.1007/978-1-4939-3721-9_11.
Recently we have explored and developed approaches imaging using confocal/two-photon microscopy, which enables simultaneous high-resolution assessment of specifically fluorescently marked cells in conjunction with structural components of the tissues visualized via harmonic generated signals. This approach uses commercially available confocal and two-photon laser microscope and automated user-interactive image analysis methods based on commercially available software packages allowing easy implementation in usual microscopy facilities.
最近，我们探索并开发了使用共聚焦/双光子显微镜进行成像的方法，该方法能够结合通过谐波产生信号可视化的组织结构成分，同时对特定荧光标记的细胞进行高分辨率评估。这种方法使用市售的共聚焦和双光子激光显微镜以及基于市售软件包的自动化用户交互式图像分析方法，便于在普通显微镜设施中轻松实施。
