Xu Xiuling, Padilla Mabel T, Lin Yong
Molecular Biology and Lung Cancer, Lovelace Respiratory Research Institute, Albuquerque, USA.
Bio Protoc. 2014 Oct 20;4(20). doi: 10.21769/bioprotoc.1264.
In cigarette smoke-induced and inflammation-associated lung cancer development, cigarette smoke extract (CSE) activates tumor necrosis factor-alpha (TNF-α) secretion from macrophages. TNF-α converting enzyme (TACE), also known as α-Secretase or ADAM17 (A Disintegrin and Metalloprotease), is a member of the ADAM family of metalloproteases. TACE mediated ectodomain shedding leads to the conversion of the inactive TNF-α precursor into the active mature pro-inflammatory cytokine. The SensoLyte 520 TACE (α-Secretase) Activity Assay Kit was used to detect TACE activity in CSE-activated macrophages. This assay is reliable, reproducible and easy to carry out in 96 well plate format.
在香烟烟雾诱导和炎症相关的肺癌发展过程中，香烟烟雾提取物（CSE）可激活巨噬细胞分泌肿瘤坏死因子-α（TNF-α）。TNF-α转换酶（TACE），也称为α-分泌酶或ADAM17（一种去整合素和金属蛋白酶），是金属蛋白酶ADAM家族的成员。TACE介导的胞外域脱落导致无活性的TNF-α前体转化为有活性的成熟促炎细胞因子。使用SensoLyte 520 TACE（α-分泌酶）活性检测试剂盒检测CSE激活的巨噬细胞中的TACE活性。该检测方法可靠、可重复，且易于以96孔板形式进行操作。
