色素靶向的光波长和强度促进蓝藻的高效光自养生长。
Pigment-targeted light wavelength and intensity promotes efficient photoautotrophic growth of Cyanobacteria.
机构信息
Department of Biological and Environmental Engineering, 226 Riley-Robb Hall, Cornell University, Ithaca, NY 14853, USA.
Department of Biological and Environmental Engineering, 226 Riley-Robb Hall, Cornell University, Ithaca, NY 14853, USA; Faculty fellow, Atkinson Center for a Sustainable Future, 200 Rice Hall, Cornell University, Ithaca, NY 14853, USA.
出版信息
Bioresour Technol. 2016 Sep;216:579-86. doi: 10.1016/j.biortech.2016.05.116. Epub 2016 May 30.
A consensus is lacking whether monochromatic rather than broad-spectrum illumination is more efficient for photosynthetic microbe production platforms. Light wavelength and intensity were tuned to pigment composition for growth of the Cyanobacterium Synechocystis PCC 6803. Phycocyanin (PC)-targeting LEDs (620nm) provided more than 6times the peak efficiency of white LEDs, with peak efficiency growth rates of 0.063h(-1) at 81μEm(-2)s(-1) and 0.039h(-1) at 126μEm(-2)s(-1) for red and white LEDs, respectively. Chlorophyll a (Chl a)-targeting LEDs (680- and 440-nm) performed poorly. Indeed, 10 times greater mass abundance was observed for PC than Chl a. PC levels did not change while Chl a levels decreased when Synechocystis transitioned from white light at 50μEm(-2)s(-1) to 250μEm(-2)s(-1) with 620nm, 680nm, or white LEDs. This work demonstrates that light wavelengths and intensity need to be optimized for each strain.
对于光合微生物生产平台而言，单色光是否比广谱光更有效，目前尚无定论。为了使蓝藻集胞藻 PCC 6803 生长，我们对光的波长和强度进行了调整，使其与色素组成相匹配。藻蓝蛋白（PC）靶向 LED（620nm）的效率比白色 LED 高出 6 倍以上，在 81μEm(-2)s(-1)的 126μEm(-2)s(-1)和 0.039h(-1)时，峰值效率增长率分别为 0.063h(-1)和 0.039h(-1)。而叶绿素 a（Chl a）靶向 LED（680nm 和 440nm）的效果则不佳。事实上，PC 的质量丰度比 Chl a 高出 10 倍。当集胞藻从 50μEm(-2)s(-1)的白光切换到 620nm、680nm 或白色 LED 的 250μEm(-2)s(-1)时，PC 水平没有变化，而 Chl a 水平下降。这项工作表明，需要针对每种菌株优化光的波长和强度。
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