Ikegami S, Kajiyama N, Ozaki Y, Ooe Y, Shimizu T, Kasahara T, Uemura D, Shioda M
a Department of Applied Biochemistry , Hiroshima University , 4-4, Kagamiyama 1-chome, Higashi-hiroshima-shi , Hiroshima 724 , Japan.
b Faculty of Liberal Arts , Shizuoka University , Ohya, Shizuoka 422 , Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(7):1007-11. doi: 10.1271/bbb.56.1007.
The effects of okadaic acid, an inhibitor of protein phosphatases types 1 and 2A, from the sponge Halichondria okadai Kadota, on the embryonic development of the starfish Asterina pectinifera, were investigated. When cultured in okadaic acid from fertilization, the embryo divided synchronously without any abnormalities such as lysis, swelling or morphological changes different from control embryos up to the 32-cell stage. However, okadaic acid prevented development before the onset of blastulation. Cytological examination showed that chromosomes condensed but did not align in a plane in the mitotic apparatus in each of the blastomeres of the treated embryo at the sixth cleavage, suggesting that this was the root cause of the arrest of further development.
研究了从冈村软海绵（Halichondria okadai Kadota）中提取的蛋白磷酸酶1型和2A型抑制剂冈田酸对多棘海盘车（Asterina pectinifera）胚胎发育的影响。从受精开始就在冈田酸中培养时，胚胎同步分裂，直至32细胞期都没有出现任何异常，如裂解、肿胀或与对照胚胎不同的形态变化。然而，冈田酸在囊胚形成开始前阻止了发育。细胞学检查表明，在第六次卵裂时，处理过的胚胎的每个卵裂球中的染色体在有丝分裂器中浓缩但未在一个平面上排列，这表明这是进一步发育停滞的根本原因。
