Liu Junwei, Wang Yong, Wu Shuchao, Zhang Peimin, Zhu Yan
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou, China.
Institute of Forensic Science and Technology of Nanjing Public Security Bureau, Nanjing, China.
J Sep Sci. 2016 Aug;39(15):2970-7. doi: 10.1002/jssc.201600463. Epub 2016 Jul 8.
In this work, poly(glycidylmethacrylate-divinylbenzene) microspheres were prepared and applied for the preparation of weak acid cation exchange stationary phases. Succinic anhydride, phthalic anhydride, and maleic anhydride were selected as carboxylation reagents to prepare three weak acid cation exchangers by direct chemical derivatization reaction without solvent or catalyst. The diameters and dispersity of the microspheres were characterized by scanning electron microscopy; the amount of accessible epoxy groups and mechanical stability were also measured. The weak acid cation exchangers were characterized by Fourier transform infrared spectroscopy; the content of carboxyl groups was measured by traditional acid base titration method. The chromatographic properties were characterized and compared by separating alkali, alkaline earth metal ions and ammonium and polar amines. The separation properties enhanced in the order of succinic anhydride, phthalic anhydride, and maleic anhydride modified poly(glycidylmethacrylate-divinylbenzene) cation exchangers.
在本工作中，制备了聚（甲基丙烯酸缩水甘油酯 - 二乙烯基苯）微球，并将其应用于制备弱酸阳离子交换固定相。选择琥珀酸酐、邻苯二甲酸酐和马来酸酐作为羧化试剂，通过无溶剂或催化剂的直接化学衍生化反应制备了三种弱酸阳离子交换剂。通过扫描电子显微镜对微球的直径和分散性进行了表征；还测量了可及环氧基团的数量和机械稳定性。通过傅里叶变换红外光谱对弱酸阳离子交换剂进行了表征；通过传统酸碱滴定法测量了羧基的含量。通过分离碱金属、碱土金属离子以及铵和极性胺对色谱性能进行了表征和比较。琥珀酸酐、邻苯二甲酸酐和马来酸酐改性的聚（甲基丙烯酸缩水甘油酯 - 二乙烯基苯）阳离子交换剂的分离性能按此顺序增强。
