Wang Yijing, Partridge Ashton, Wu Yinqiu
The New Zealand Institute for Plant & Food Research, Sandringham, Auckland 1025, New Zealand; The University of Auckland, Auckland 1010, New Zealand.
The University of Auckland, Auckland 1010, New Zealand; MSL, Rosedale, Auckland 0632, New Zealand.
Anal Biochem. 2016 Sep 1;508:46-9. doi: 10.1016/j.ab.2016.05.027. Epub 2016 Jun 8.
Functionalization of a gold surface is usually accomplished by covalent binding via self-assembled monolayers (SAMs) on the gold surface, followed by attachment of flexible polymeric linker layers such as dextran hydrogels. However, these techniques require multiple steps and also have nonspecific interactions and steric problems. In this study, a self-assembled carboxylated terthiophene monolayer was formed onto a gold surface to create a sensitive and stable surface plasmon resonance (SPR) biosensing system. Compared with a commercial carboxymethyl dextran chip (CM5), the terthiophene SAM surface provided more than six times more antibody-binding signals and nearly three times the SPR assay sensitivity for progesterone (P4).
金表面的功能化通常通过在金表面上经由自组装单分子层（SAMs）进行共价结合来实现，随后连接柔性聚合物连接层，如葡聚糖水凝胶。然而，这些技术需要多个步骤，并且还存在非特异性相互作用和空间位阻问题。在本研究中，在金表面形成了自组装的羧基化三联噻吩单分子层，以创建一个灵敏且稳定的表面等离子体共振（SPR）生物传感系统。与商业羧甲基葡聚糖芯片（CM5）相比，三联噻吩SAM表面提供的抗体结合信号多出六倍以上，对孕酮（P4）的SPR检测灵敏度几乎高出三倍。
