Sil Susmita, Ghosh Arijit, Ghosh Tusharkanti
Neurophysiology Laboratory, Department of Physiology, University College of Science and Technology, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata 700009, West Bengal, India.
Neurophysiology Laboratory, Department of Physiology, University College of Science and Technology, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata 700009, West Bengal, India.
Brain Res. 2016 Sep 1;1646:278-286. doi: 10.1016/j.brainres.2016.05.052. Epub 2016 Jun 8.
The neurodegeneration in AD patients may be associated with changes of peripheral immune responses. Some peripheral immune responses are altered due to neuroinflammation in colchicine induced AD (cAD) rats. The leaky blood brain barrier (BBB) in cAD-rats may be involved in inducing peripheral inflammation, though there is no report in this regard. Therefore, the present study was designed to investigate the role of BBB in cADrats by altering the BBB in a time dependent manner with injection (i.v.) of mannitol (BBB opener). The inflammatory markers in the brain and serum along with the peripheral immune responses were measured after 30 and 60min of mannitol injection in cAD rats. The results showed higher inflammatory markers in the hippocampus and serum along with alterations in peripheral immune parameters in cAD rats. Although the hippocampal inflammatory markers did not further change after mannitol injection in cAD rats, the serum inflammatory markers and peripheral immune responses were altered and these changes were greater after 60min than that of 30min of mannitol injection. The present study shows that the peripheral immune responses in cAD rats after 30 and 60min of mannitol injection are related to magnitude of impairment of BBB in these conditions. It can be concluded from this study that impairment of BBB in cAD rats is related to the changes of peripheral immune responses observed in that condition.
阿尔茨海默病（AD）患者的神经退行性变可能与外周免疫反应的变化有关。在秋水仙碱诱导的AD（cAD）大鼠中，一些外周免疫反应因神经炎症而改变。尽管尚无这方面的报道，但cAD大鼠中血脑屏障（BBB）的渗漏可能参与诱导外周炎症。因此，本研究旨在通过静脉注射甘露醇（BBB开放剂）以时间依赖性方式改变BBB，来研究BBB在cAD大鼠中的作用。在给cAD大鼠注射甘露醇30分钟和60分钟后，测量大脑和血清中的炎症标志物以及外周免疫反应。结果显示，cAD大鼠海马体和血清中的炎症标志物升高，外周免疫参数也发生改变。虽然在cAD大鼠中注射甘露醇后海马体炎症标志物没有进一步变化，但血清炎症标志物和外周免疫反应发生了改变，且在注射甘露醇60分钟后的变化比30分钟时更大。本研究表明，在注射甘露醇30分钟和60分钟后，cAD大鼠的外周免疫反应与这些情况下BBB的损伤程度有关。从这项研究可以得出结论，cAD大鼠中BBB的损伤与在该情况下观察到的外周免疫反应变化有关。
