School of Life Science, Liaoning Normal University, Dalian 116081, China; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
School of Life Science, Liaoning Normal University, Dalian 116081, China.
Bioresour Technol. 2016 Sep;216:684-90. doi: 10.1016/j.biortech.2016.06.009. Epub 2016 Jun 3.
Magnetically immobilized cells of Comamonas sp. JB coupling with electrode reaction was developed to enhance the treatment efficiency of coking wastewater containing phenol, carbazole (CA), dibenzofuran (DBF), and dibenzothiophene (DBT). The pair of graphite plate-stainless iron mesh electrodes was chosen as the most suitable electrodes. Magnetically immobilized cells coupling with graphite plate-stainless iron mesh electrodes (coupling system) exhibited high degradation activity for all the compounds, which were significantly higher than the sum by single magnetically immobilized cells and electrode reaction at the optimal voltage. Recycling experiments demonstrated that the degradation activity of coupling system increased gradually during eight recycles, indicating that there was a coupling effect between the biodegradation and electrode reaction. Phenol hydroxylase and qPCR assays confirmed that appropriate electrical stimulation could improve phenol hydroxylase activity and promote cells growth. Toxicity assessment suggested the treatment of the coking wastewater by coupling system led to less toxicity than untreated wastewater.
采用磁固定 Comamonas sp. JB 细胞与电极反应耦合的方法，提高了含苯酚、咔唑（CA）、二苯并呋喃（DBF）和二苯并噻吩（DBT）的焦化废水的处理效率。选择石墨板-不锈钢网电极对作为最适合的电极。磁固定细胞与石墨板-不锈钢网电极耦合（耦合系统）对所有化合物均表现出高降解活性，明显高于最佳电压下单一磁固定细胞和电极反应的总和。回收实验表明，在 8 次循环中，耦合系统的降解活性逐渐增加，表明生物降解和电极反应之间存在耦合效应。苯酚羟化酶和 qPCR 检测证实，适当的电刺激可以提高苯酚羟化酶的活性并促进细胞生长。毒性评估表明，耦合系统处理焦化废水的毒性比未经处理的废水低。
