Villa-Osaba Alicia, Gahete Manuel D, Cordoba-Chacon José, de Lecea Luis, Castaño Justo P, Luque Raúl M
Maimonides Institute of Biomedical Research of Cordoba (IMIBIC), Spain; Department of Cell Biology, Physiology and Immunology, University of Córdoba, Spain; Hospital Universitario Reina Sofía (HURS), Spain; CIBERobn, Córdoba, Spain; ceiA3, Córdoba, Spain.
Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Palo Alto, CA, USA.
Mol Cell Endocrinol. 2016 Oct 15;434:14-24. doi: 10.1016/j.mce.2016.06.014. Epub 2016 Jun 9.
Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are essential factors in mammary-gland (MG) development and are altered during fasting. However, no studies have investigated the alterations in the expression of GH/IGF-I and its regulatory systems (somatostatin/cortistatin and ghrelin) in MG during fasting. Therefore, this study was aimed at characterizing the regulation of GH/IGF-I/somatostatin/cortistatin/ghrelin-systems expression in MG of fasted female-mice (compared to fed-controls) and the influence of endogenous-cortistatin (using cortistatin-knockouts). Fasting decreased IGF-I while increased IGF-I/Insulin-receptors expression in MGs. Fasting provoked an increase in GH expression that might be associated to enhanced ghrelin-variants/ghrelin-O-acyl-transferase enzyme expression, while an upregulation of somatostatin-receptors was observed. However, cortistatin-knockouts mice showed a decrease in GH and somatostatin receptor-subtypes expression. Altogether, we demonstrate that GH/IGF-I, somatostatin/cortistatin and ghrelin systems expression is altered in MG during fasting, suggesting a relevant role in coordinating its response to metabolic stress, wherein endogenous cortistatin might be essential for an appropriate response.
生长激素（GH）和胰岛素样生长因子-I（IGF-I）是乳腺（MG）发育的关键因素，且在禁食期间会发生变化。然而，尚无研究探讨禁食期间MG中GH/IGF-I及其调节系统（生长抑素/皮质抑素和胃饥饿素）表达的改变。因此，本研究旨在表征禁食雌性小鼠（与喂食对照相比）MG中GH/IGF-I/生长抑素/皮质抑素/胃饥饿素系统表达的调节以及内源性皮质抑素（使用皮质抑素基因敲除小鼠）的影响。禁食使MG中的IGF-I减少，而IGF-I/胰岛素受体表达增加。禁食引发GH表达增加，这可能与胃饥饿素变体/胃饥饿素-O-酰基转移酶表达增强有关，同时观察到生长抑素受体上调。然而，皮质抑素基因敲除小鼠的GH和生长抑素受体亚型表达降低。总之，我们证明禁食期间MG中GH/IGF-I、生长抑素/皮质抑素和胃饥饿素系统表达发生改变，表明其在协调对代谢应激的反应中具有重要作用，其中内源性皮质抑素可能是适当反应所必需的。
